The TAGteam motif facilitates binding of 21 sequence-specific transcription factors in the Drosophila embryo

Rahul Satija, Robert K. Bradley

Research output: Contribution to journalArticle

Abstract

Highly overlapping patterns of genome-wide binding of many distinct transcription factors have been observed in worms, insects, and mammals, but the origins and consequences of this overlapping binding remain unclear. While analyzing chromatin immunoprecipitation data sets from 21 sequence-specific transcription factors active in the Drosophila embryo, we found that binding of all factors exhibits a dose-dependent relationship with "TAGteam" sequence motifs bound by the zinc finger protein Vielfaltig, also known as Zelda, a recently discovered activator of the zygotic genome. TAGteam motifs are present and well conserved in highly bound regions, and are associated with transcription factor binding even in the absence of canonical recognition motifs for these factors. Furthermore, levels of binding in promoters and enhancers of zygotically transcribed genes are correlated with RNA polymerase II occupancy and gene expression levels. Our results suggest that Vielfaltig acts as a master regulator of early development by facilitating the genome-wide establishment of overlapping patterns of binding of diverse transcription factors that drive global gene expression.

Original languageEnglish (US)
Pages (from-to)656-665
Number of pages10
JournalGenome Research
Volume22
Issue number4
DOIs
StatePublished - Apr 2012

Fingerprint

Drosophila
Transcription Factors
Embryonic Structures
Genome
Gene Expression
RNA Polymerase II
Chromatin Immunoprecipitation
Zinc Fingers
Insects
Mammals
Genes
Proteins

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

The TAGteam motif facilitates binding of 21 sequence-specific transcription factors in the Drosophila embryo. / Satija, Rahul; Bradley, Robert K.

In: Genome Research, Vol. 22, No. 4, 04.2012, p. 656-665.

Research output: Contribution to journalArticle

@article{8bd286b48a1b4a57b9a8b7a987797094,
title = "The TAGteam motif facilitates binding of 21 sequence-specific transcription factors in the Drosophila embryo",
abstract = "Highly overlapping patterns of genome-wide binding of many distinct transcription factors have been observed in worms, insects, and mammals, but the origins and consequences of this overlapping binding remain unclear. While analyzing chromatin immunoprecipitation data sets from 21 sequence-specific transcription factors active in the Drosophila embryo, we found that binding of all factors exhibits a dose-dependent relationship with {"}TAGteam{"} sequence motifs bound by the zinc finger protein Vielfaltig, also known as Zelda, a recently discovered activator of the zygotic genome. TAGteam motifs are present and well conserved in highly bound regions, and are associated with transcription factor binding even in the absence of canonical recognition motifs for these factors. Furthermore, levels of binding in promoters and enhancers of zygotically transcribed genes are correlated with RNA polymerase II occupancy and gene expression levels. Our results suggest that Vielfaltig acts as a master regulator of early development by facilitating the genome-wide establishment of overlapping patterns of binding of diverse transcription factors that drive global gene expression.",
author = "Rahul Satija and Bradley, {Robert K.}",
year = "2012",
month = "4",
doi = "10.1101/gr.130682.111",
language = "English (US)",
volume = "22",
pages = "656--665",
journal = "Genome Research",
issn = "1088-9051",
publisher = "Cold Spring Harbor Laboratory Press",
number = "4",

}

TY - JOUR

T1 - The TAGteam motif facilitates binding of 21 sequence-specific transcription factors in the Drosophila embryo

AU - Satija, Rahul

AU - Bradley, Robert K.

PY - 2012/4

Y1 - 2012/4

N2 - Highly overlapping patterns of genome-wide binding of many distinct transcription factors have been observed in worms, insects, and mammals, but the origins and consequences of this overlapping binding remain unclear. While analyzing chromatin immunoprecipitation data sets from 21 sequence-specific transcription factors active in the Drosophila embryo, we found that binding of all factors exhibits a dose-dependent relationship with "TAGteam" sequence motifs bound by the zinc finger protein Vielfaltig, also known as Zelda, a recently discovered activator of the zygotic genome. TAGteam motifs are present and well conserved in highly bound regions, and are associated with transcription factor binding even in the absence of canonical recognition motifs for these factors. Furthermore, levels of binding in promoters and enhancers of zygotically transcribed genes are correlated with RNA polymerase II occupancy and gene expression levels. Our results suggest that Vielfaltig acts as a master regulator of early development by facilitating the genome-wide establishment of overlapping patterns of binding of diverse transcription factors that drive global gene expression.

AB - Highly overlapping patterns of genome-wide binding of many distinct transcription factors have been observed in worms, insects, and mammals, but the origins and consequences of this overlapping binding remain unclear. While analyzing chromatin immunoprecipitation data sets from 21 sequence-specific transcription factors active in the Drosophila embryo, we found that binding of all factors exhibits a dose-dependent relationship with "TAGteam" sequence motifs bound by the zinc finger protein Vielfaltig, also known as Zelda, a recently discovered activator of the zygotic genome. TAGteam motifs are present and well conserved in highly bound regions, and are associated with transcription factor binding even in the absence of canonical recognition motifs for these factors. Furthermore, levels of binding in promoters and enhancers of zygotically transcribed genes are correlated with RNA polymerase II occupancy and gene expression levels. Our results suggest that Vielfaltig acts as a master regulator of early development by facilitating the genome-wide establishment of overlapping patterns of binding of diverse transcription factors that drive global gene expression.

UR - http://www.scopus.com/inward/record.url?scp=84859528845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859528845&partnerID=8YFLogxK

U2 - 10.1101/gr.130682.111

DO - 10.1101/gr.130682.111

M3 - Article

VL - 22

SP - 656

EP - 665

JO - Genome Research

JF - Genome Research

SN - 1088-9051

IS - 4

ER -