The subcellular distribution of nitric oxide synthase relative to the NR1 subunit of NMDA receptors in the cerebral cortex

Chiye Aoki, D. S. Bredt, S. Fenstemaker, M. Lubin

Research output: Contribution to journalArticle

Abstract

Results from several electrophysiological studies predict that the neuronal NO-synthesizing enzyme, nNOS, resides within spines formed by pyramid-to-pyramid axo-spinous synaptic junctions of the cortex. On the other hand, light microscopic neuroanatomical detection of nNOS within pyramidal neurons has been difficult, suggesting that these neurons contain nNOS at levels below threshold for detection. Our results obtained by electron microscopic immunocytochemistry indicate that nNOS occurs within spiny neurons, such as those of pyramidal neurons, albeit discretely within their spines. Dual electron microscopic immunocytochemistry, whereby antigenic sites to the NR1 subunit of NMDA receptors are probed simultaneously with sites immunoreactive for nNOS, reveals that some, although not all, nNOS within spines co-exist with NR1 subunits. Additionally, immunoreactivity for the NR1 subunit is detectable within nNOS-axons, indicating that NO may be generated in response to axo-axonic interactions with glutamatergic axons in the vicinity and independently of action potential propagation. Immunoreactivity for NR1 subunits within axons (with or without nNOS- immunoreactivity) may additionally serve to confer receptivity of these axons to NO generated coincidentally with activity. Analysis of the visual cortex of monocular adult animals indicates that the level of nNOS within neurites is dependent on chronic activity levels of the surrounding neuropil and independent of somatic input level. Together, these findings point to plasticity of nNOS neurons within adult brain tissue, involving regulation of subcellular nNOS distribution.

Original languageEnglish (US)
Pages (from-to)83-97
Number of pages15
JournalProgress in Brain Research
Volume118
StatePublished - 1998

Fingerprint

Nitric Oxide Synthase
Cerebral Cortex
Axons
Spine
Pyramidal Cells
Neurons
Immunohistochemistry
Electrons
Nitric Oxide Synthase Type I
Neuropil
Neurites
Visual Cortex
Action Potentials
Light
NR1 NMDA receptor
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The subcellular distribution of nitric oxide synthase relative to the NR1 subunit of NMDA receptors in the cerebral cortex. / Aoki, Chiye; Bredt, D. S.; Fenstemaker, S.; Lubin, M.

In: Progress in Brain Research, Vol. 118, 1998, p. 83-97.

Research output: Contribution to journalArticle

@article{ed59814ef93441e1a335af739e54a764,
title = "The subcellular distribution of nitric oxide synthase relative to the NR1 subunit of NMDA receptors in the cerebral cortex",
abstract = "Results from several electrophysiological studies predict that the neuronal NO-synthesizing enzyme, nNOS, resides within spines formed by pyramid-to-pyramid axo-spinous synaptic junctions of the cortex. On the other hand, light microscopic neuroanatomical detection of nNOS within pyramidal neurons has been difficult, suggesting that these neurons contain nNOS at levels below threshold for detection. Our results obtained by electron microscopic immunocytochemistry indicate that nNOS occurs within spiny neurons, such as those of pyramidal neurons, albeit discretely within their spines. Dual electron microscopic immunocytochemistry, whereby antigenic sites to the NR1 subunit of NMDA receptors are probed simultaneously with sites immunoreactive for nNOS, reveals that some, although not all, nNOS within spines co-exist with NR1 subunits. Additionally, immunoreactivity for the NR1 subunit is detectable within nNOS-axons, indicating that NO may be generated in response to axo-axonic interactions with glutamatergic axons in the vicinity and independently of action potential propagation. Immunoreactivity for NR1 subunits within axons (with or without nNOS- immunoreactivity) may additionally serve to confer receptivity of these axons to NO generated coincidentally with activity. Analysis of the visual cortex of monocular adult animals indicates that the level of nNOS within neurites is dependent on chronic activity levels of the surrounding neuropil and independent of somatic input level. Together, these findings point to plasticity of nNOS neurons within adult brain tissue, involving regulation of subcellular nNOS distribution.",
author = "Chiye Aoki and Bredt, {D. S.} and S. Fenstemaker and M. Lubin",
year = "1998",
language = "English (US)",
volume = "118",
pages = "83--97",
journal = "Progress in Brain Research",
issn = "0079-6123",
publisher = "Elsevier",

}

TY - JOUR

T1 - The subcellular distribution of nitric oxide synthase relative to the NR1 subunit of NMDA receptors in the cerebral cortex

AU - Aoki, Chiye

AU - Bredt, D. S.

AU - Fenstemaker, S.

AU - Lubin, M.

PY - 1998

Y1 - 1998

N2 - Results from several electrophysiological studies predict that the neuronal NO-synthesizing enzyme, nNOS, resides within spines formed by pyramid-to-pyramid axo-spinous synaptic junctions of the cortex. On the other hand, light microscopic neuroanatomical detection of nNOS within pyramidal neurons has been difficult, suggesting that these neurons contain nNOS at levels below threshold for detection. Our results obtained by electron microscopic immunocytochemistry indicate that nNOS occurs within spiny neurons, such as those of pyramidal neurons, albeit discretely within their spines. Dual electron microscopic immunocytochemistry, whereby antigenic sites to the NR1 subunit of NMDA receptors are probed simultaneously with sites immunoreactive for nNOS, reveals that some, although not all, nNOS within spines co-exist with NR1 subunits. Additionally, immunoreactivity for the NR1 subunit is detectable within nNOS-axons, indicating that NO may be generated in response to axo-axonic interactions with glutamatergic axons in the vicinity and independently of action potential propagation. Immunoreactivity for NR1 subunits within axons (with or without nNOS- immunoreactivity) may additionally serve to confer receptivity of these axons to NO generated coincidentally with activity. Analysis of the visual cortex of monocular adult animals indicates that the level of nNOS within neurites is dependent on chronic activity levels of the surrounding neuropil and independent of somatic input level. Together, these findings point to plasticity of nNOS neurons within adult brain tissue, involving regulation of subcellular nNOS distribution.

AB - Results from several electrophysiological studies predict that the neuronal NO-synthesizing enzyme, nNOS, resides within spines formed by pyramid-to-pyramid axo-spinous synaptic junctions of the cortex. On the other hand, light microscopic neuroanatomical detection of nNOS within pyramidal neurons has been difficult, suggesting that these neurons contain nNOS at levels below threshold for detection. Our results obtained by electron microscopic immunocytochemistry indicate that nNOS occurs within spiny neurons, such as those of pyramidal neurons, albeit discretely within their spines. Dual electron microscopic immunocytochemistry, whereby antigenic sites to the NR1 subunit of NMDA receptors are probed simultaneously with sites immunoreactive for nNOS, reveals that some, although not all, nNOS within spines co-exist with NR1 subunits. Additionally, immunoreactivity for the NR1 subunit is detectable within nNOS-axons, indicating that NO may be generated in response to axo-axonic interactions with glutamatergic axons in the vicinity and independently of action potential propagation. Immunoreactivity for NR1 subunits within axons (with or without nNOS- immunoreactivity) may additionally serve to confer receptivity of these axons to NO generated coincidentally with activity. Analysis of the visual cortex of monocular adult animals indicates that the level of nNOS within neurites is dependent on chronic activity levels of the surrounding neuropil and independent of somatic input level. Together, these findings point to plasticity of nNOS neurons within adult brain tissue, involving regulation of subcellular nNOS distribution.

UR - http://www.scopus.com/inward/record.url?scp=0031774289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031774289&partnerID=8YFLogxK

M3 - Article

VL - 118

SP - 83

EP - 97

JO - Progress in Brain Research

JF - Progress in Brain Research

SN - 0079-6123

ER -