Abstract
The soluble glycoprotein Gs of vesicular stomatitis virus (VSV), at approximately 104 molecules per cell, cells for lysis by clones of CD4+ cytolytic T lymphocytes (CTL). In addition to lysis, the clones responded by proliferation and interleukin-2 release. Targets sensitized by Gs competed effectively with VSV-infected cell for recognition. Immune cytolysis by CD4+ CTLs was restricted by class II major histocompatibility complex (MHC) antigens and was specific to VSV. The specific class II MHC antigen which was restricting for each clone remained the same whether the targets were sensitized by infection with VSV or by exogenously added souble antigen. Sensitization by Gs appeared to require prior processing because the antigen-presenting cells that were fixed prior to exposure to Gs filed to be recognized by te CTL clones. The high efficiency of this uptake and processing was suggested by the inability of Gs at concentrations up to 107 per cell t block superinfection by VSV or to effect the RNA-synthetic machinery of uninfected cells. Also, Gs failed to hemolyze sheep erythrocytes when there was hemolysis by virions or an amin-terminal peptide of the VSV glycoprotein. extrapolation of these results to viral deseases was possible because solube viral glycoproteins were naturally synthesized during many viral infections and class II MHC antigens were inducible in cells of nonlymphoid origin. Therefore, CD4+ CTLs may be important participants in increasing virus-induced pathology, especially among adjacent uninfected cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3810-3816 |
| Number of pages | 7 |
| Journal | Journal of Virology |
| Volume | 64 |
| Issue number | 8 |
| State | Published - 1990 |
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ASJC Scopus subject areas
- Immunology
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The soluble viral glycoprotein of vesicular stomatitis virus efficiently sensitizes target cells for lysis by CD4+ T lymphocytes. / Browning, Michael; Reiss, Carol; Huang, Alice S.
In: Journal of Virology, Vol. 64, No. 8, 1990, p. 3810-3816.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The soluble viral glycoprotein of vesicular stomatitis virus efficiently sensitizes target cells for lysis by CD4+ T lymphocytes
AU - Browning, Michael
AU - Reiss, Carol
AU - Huang, Alice S.
PY - 1990
Y1 - 1990
N2 - The soluble glycoprotein Gs of vesicular stomatitis virus (VSV), at approximately 104 molecules per cell, cells for lysis by clones of CD4+ cytolytic T lymphocytes (CTL). In addition to lysis, the clones responded by proliferation and interleukin-2 release. Targets sensitized by Gs competed effectively with VSV-infected cell for recognition. Immune cytolysis by CD4+ CTLs was restricted by class II major histocompatibility complex (MHC) antigens and was specific to VSV. The specific class II MHC antigen which was restricting for each clone remained the same whether the targets were sensitized by infection with VSV or by exogenously added souble antigen. Sensitization by Gs appeared to require prior processing because the antigen-presenting cells that were fixed prior to exposure to Gs filed to be recognized by te CTL clones. The high efficiency of this uptake and processing was suggested by the inability of Gs at concentrations up to 107 per cell t block superinfection by VSV or to effect the RNA-synthetic machinery of uninfected cells. Also, Gs failed to hemolyze sheep erythrocytes when there was hemolysis by virions or an amin-terminal peptide of the VSV glycoprotein. extrapolation of these results to viral deseases was possible because solube viral glycoproteins were naturally synthesized during many viral infections and class II MHC antigens were inducible in cells of nonlymphoid origin. Therefore, CD4+ CTLs may be important participants in increasing virus-induced pathology, especially among adjacent uninfected cells.
AB - The soluble glycoprotein Gs of vesicular stomatitis virus (VSV), at approximately 104 molecules per cell, cells for lysis by clones of CD4+ cytolytic T lymphocytes (CTL). In addition to lysis, the clones responded by proliferation and interleukin-2 release. Targets sensitized by Gs competed effectively with VSV-infected cell for recognition. Immune cytolysis by CD4+ CTLs was restricted by class II major histocompatibility complex (MHC) antigens and was specific to VSV. The specific class II MHC antigen which was restricting for each clone remained the same whether the targets were sensitized by infection with VSV or by exogenously added souble antigen. Sensitization by Gs appeared to require prior processing because the antigen-presenting cells that were fixed prior to exposure to Gs filed to be recognized by te CTL clones. The high efficiency of this uptake and processing was suggested by the inability of Gs at concentrations up to 107 per cell t block superinfection by VSV or to effect the RNA-synthetic machinery of uninfected cells. Also, Gs failed to hemolyze sheep erythrocytes when there was hemolysis by virions or an amin-terminal peptide of the VSV glycoprotein. extrapolation of these results to viral deseases was possible because solube viral glycoproteins were naturally synthesized during many viral infections and class II MHC antigens were inducible in cells of nonlymphoid origin. Therefore, CD4+ CTLs may be important participants in increasing virus-induced pathology, especially among adjacent uninfected cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025286144&partnerID=8YFLogxK
M3 - Article
C2 - 2164598
AN - SCOPUS:0025286144
VL - 64
SP - 3810
EP - 3816
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 8
ER -