The soluble viral glycoprotein of vesicular stomatitis virus efficiently sensitizes target cells for lysis by CD4+ T lymphocytes

Michael Browning, Carol Reiss, Alice S. Huang

Research output: Contribution to journalArticle

Abstract

The soluble glycoprotein Gs of vesicular stomatitis virus (VSV), at approximately 104 molecules per cell, cells for lysis by clones of CD4+ cytolytic T lymphocytes (CTL). In addition to lysis, the clones responded by proliferation and interleukin-2 release. Targets sensitized by Gs competed effectively with VSV-infected cell for recognition. Immune cytolysis by CD4+ CTLs was restricted by class II major histocompatibility complex (MHC) antigens and was specific to VSV. The specific class II MHC antigen which was restricting for each clone remained the same whether the targets were sensitized by infection with VSV or by exogenously added souble antigen. Sensitization by Gs appeared to require prior processing because the antigen-presenting cells that were fixed prior to exposure to Gs filed to be recognized by te CTL clones. The high efficiency of this uptake and processing was suggested by the inability of Gs at concentrations up to 107 per cell t block superinfection by VSV or to effect the RNA-synthetic machinery of uninfected cells. Also, Gs failed to hemolyze sheep erythrocytes when there was hemolysis by virions or an amin-terminal peptide of the VSV glycoprotein. extrapolation of these results to viral deseases was possible because solube viral glycoproteins were naturally synthesized during many viral infections and class II MHC antigens were inducible in cells of nonlymphoid origin. Therefore, CD4+ CTLs may be important participants in increasing virus-induced pathology, especially among adjacent uninfected cells.

Original languageEnglish (US)
Pages (from-to)3810-3816
Number of pages7
JournalJournal of Virology
Volume64
Issue number8
StatePublished - 1990

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Vesiculovirus
Vesicular Stomatitis
glycoproteins
Glycoproteins
T-lymphocytes
Viruses
T-Lymphocytes
Histocompatibility Antigens Class II
major histocompatibility complex
Clone Cells
Major Histocompatibility Complex
clones
antigens
cells
cytolysis
Superinfection
antigen-presenting cells
hemolysis
Antigen-Presenting Cells
Virus Diseases

ASJC Scopus subject areas

  • Immunology

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The soluble viral glycoprotein of vesicular stomatitis virus efficiently sensitizes target cells for lysis by CD4+ T lymphocytes. / Browning, Michael; Reiss, Carol; Huang, Alice S.

In: Journal of Virology, Vol. 64, No. 8, 1990, p. 3810-3816.

Research output: Contribution to journalArticle

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