The sodium bicarbonate cotransporter (NBCe1) is essential for normal development of mouse dentition

Rodrigo Lacruz, Antonio Nanci, Shane N. White, Xin Wen, HongJun Wang, Sylvia F. Zalzal, Vivian Q. Luong, Verna L. Schuetter, Peter S. Conti, Ira Kurtz, Michael L. Paine

Research output: Contribution to journalArticle

Abstract

Proximal renal tubular acidosis (pRTA) is a syndrome caused by abnormal proximal tubule reabsorption of bicarbonate resulting in metabolic acidosis. Patients with mutations to the SLC4A4 gene (coding for the sodium bicarbonate cotransporter NBCe1), have pRTA, growth delay, ocular defects, and enamel abnormalities. In an earlier report, we provided the first evidence that enamel cells, the ameloblasts, express NBCe1 in a polarized fashion, thereby contributing to trans-cellular bicarbonate transport. To determine whether NBCe1 plays a critical role in enamel development, we studied the expression of NBCe1 at various stages of enamel formation in wild-type mice and characterized the biophysical properties of enamel in NBCe1-/- animals. The enamel of NBCe1-/- animals was extremely hypomineralized and weak with an abnormal prismatic architecture. The expression profile of amelogenin, a known enamel-specific gene, was not altered in NBCe1-/- animals. Our results show for the first time that NBCe1 expression is required for the development of normal enamel. This study provides a mechanistic model to account for enamel abnormalities in certain patients with pRTA.

Original languageEnglish (US)
Pages (from-to)24432-24438
Number of pages7
JournalJournal of Biological Chemistry
Volume285
Issue number32
DOIs
StatePublished - Aug 6 2010

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Sodium-Bicarbonate Symporters
Dentition
Enamels
Dental Enamel
Renal Tubular Acidosis
Animals
Bicarbonates
Genes
Amelogenin
Ameloblasts
Acidosis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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The sodium bicarbonate cotransporter (NBCe1) is essential for normal development of mouse dentition. / Lacruz, Rodrigo; Nanci, Antonio; White, Shane N.; Wen, Xin; Wang, HongJun; Zalzal, Sylvia F.; Luong, Vivian Q.; Schuetter, Verna L.; Conti, Peter S.; Kurtz, Ira; Paine, Michael L.

In: Journal of Biological Chemistry, Vol. 285, No. 32, 06.08.2010, p. 24432-24438.

Research output: Contribution to journalArticle

Lacruz, R, Nanci, A, White, SN, Wen, X, Wang, H, Zalzal, SF, Luong, VQ, Schuetter, VL, Conti, PS, Kurtz, I & Paine, ML 2010, 'The sodium bicarbonate cotransporter (NBCe1) is essential for normal development of mouse dentition', Journal of Biological Chemistry, vol. 285, no. 32, pp. 24432-24438. https://doi.org/10.1074/jbc.M110.115188
Lacruz, Rodrigo ; Nanci, Antonio ; White, Shane N. ; Wen, Xin ; Wang, HongJun ; Zalzal, Sylvia F. ; Luong, Vivian Q. ; Schuetter, Verna L. ; Conti, Peter S. ; Kurtz, Ira ; Paine, Michael L. / The sodium bicarbonate cotransporter (NBCe1) is essential for normal development of mouse dentition. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 32. pp. 24432-24438.
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