The role of insulin receptor signaling in zebrafish embryogenesis

Yuka Toyoshima, Christopher Monson, Cunming Duan, Yingjie Wu, Chuan Gao, Shoshana Yakar, Kirsten Sadler Edepli, Derek LeRoith

Research output: Contribution to journalArticle

Abstract

Insulin receptor (IR) signaling is considered to be important in growth and development in addition to its major role in metabolic homeostasis. The metabolic role of insulin in carbohydrate and lipid metabolism is extensively studied. In contrast, the role of IR activation during embryogenesis is less understood. To address this, we examined the function of the IR during zebrafish development. Zebrafish express two isoforms of IR (insra and insrb). Both isoforms were cloned and show high homology to the human insulin receptor and can functionally substitute for the human IR in fibroblasts derived from insr gene-deleted mice. Gene expression studies reveal that these receptors are expressed at moderate levels in the central nervous system during development. Morpholino-mediated selective knockdown of each of the IR isoforms causes growth retardation and profound morphogenetic defects in the brain and eye. These results clearly demonstrate that IR signaling plays essential roles in vertebrate embryogenesis and growth.

Original languageEnglish (US)
Pages (from-to)5996-6005
Number of pages10
JournalEndocrinology
Volume149
Issue number12
DOIs
StatePublished - Dec 2008

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Insulin Receptor
Zebrafish
Embryonic Development
Protein Isoforms
Morpholinos
Carbohydrate Metabolism
Growth
Growth and Development
Lipid Metabolism
Vertebrates
Homeostasis
Central Nervous System
Fibroblasts
Insulin
Gene Expression
Brain
Genes
human INSR protein

ASJC Scopus subject areas

  • Endocrinology

Cite this

Toyoshima, Y., Monson, C., Duan, C., Wu, Y., Gao, C., Yakar, S., ... LeRoith, D. (2008). The role of insulin receptor signaling in zebrafish embryogenesis. Endocrinology, 149(12), 5996-6005. https://doi.org/10.1210/en.2008-0329

The role of insulin receptor signaling in zebrafish embryogenesis. / Toyoshima, Yuka; Monson, Christopher; Duan, Cunming; Wu, Yingjie; Gao, Chuan; Yakar, Shoshana; Sadler Edepli, Kirsten; LeRoith, Derek.

In: Endocrinology, Vol. 149, No. 12, 12.2008, p. 5996-6005.

Research output: Contribution to journalArticle

Toyoshima, Y, Monson, C, Duan, C, Wu, Y, Gao, C, Yakar, S, Sadler Edepli, K & LeRoith, D 2008, 'The role of insulin receptor signaling in zebrafish embryogenesis', Endocrinology, vol. 149, no. 12, pp. 5996-6005. https://doi.org/10.1210/en.2008-0329
Toyoshima Y, Monson C, Duan C, Wu Y, Gao C, Yakar S et al. The role of insulin receptor signaling in zebrafish embryogenesis. Endocrinology. 2008 Dec;149(12):5996-6005. https://doi.org/10.1210/en.2008-0329
Toyoshima, Yuka ; Monson, Christopher ; Duan, Cunming ; Wu, Yingjie ; Gao, Chuan ; Yakar, Shoshana ; Sadler Edepli, Kirsten ; LeRoith, Derek. / The role of insulin receptor signaling in zebrafish embryogenesis. In: Endocrinology. 2008 ; Vol. 149, No. 12. pp. 5996-6005.
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