The role of context on α-helix stabilization

Host-guest analysis in a mixed background peptide model

Jianxin Yang, Erik J. Spek, Youxiang Gong, Hongxing Zhou, Neville R. Kallenbach

Research output: Contribution to journalArticle

Abstract

The helix content of a series of peptides containing single substitutions of the 20 natural amino acids in a new designed host sequence, succinyl-YSEEEEKAKKAXAEEAEKKKK-NH2, has been determined using CD spectroscopy. This host is related to one previously studied, in which triple amino acid substitutions were introduced into a background of Glu-Lys blocks completely lacking alanine. The resulting free energies show that only Ala and Glu- prove to be helix stabilizing, while all other side chains are neutral or destabilizing. This agrees with results from studies of alanine- rich peptide models, but not the previous Glu-Lys block oligomers in which Leu and Met also stabilize helix. The helix propensity scale derived from the previous block oligomers correlated well with the frequencies of occurrence of different side chains in helical sequences of proteins, whereas the values from the present series do not. The role of context in determining scales of helix propensity values is discussed, and the ability of algorithms designed to predict helix structure from sequence is compared.

Original languageEnglish (US)
Pages (from-to)1264-1272
Number of pages9
JournalProtein Science
Volume6
Issue number6
StatePublished - Jun 1997

Fingerprint

Oligomers
Alanine
Substitution reactions
Stabilization
Amino Acids
Peptides
Amino Acid Substitution
Free energy
Spectrum Analysis
Spectroscopy
Proteins

Keywords

  • α-Helix
  • CD
  • Helix propensities
  • Helix stability
  • NMR
  • Secondary structure
  • Synthetic peptide

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yang, J., Spek, E. J., Gong, Y., Zhou, H., & Kallenbach, N. R. (1997). The role of context on α-helix stabilization: Host-guest analysis in a mixed background peptide model. Protein Science, 6(6), 1264-1272.

The role of context on α-helix stabilization : Host-guest analysis in a mixed background peptide model. / Yang, Jianxin; Spek, Erik J.; Gong, Youxiang; Zhou, Hongxing; Kallenbach, Neville R.

In: Protein Science, Vol. 6, No. 6, 06.1997, p. 1264-1272.

Research output: Contribution to journalArticle

Yang, J, Spek, EJ, Gong, Y, Zhou, H & Kallenbach, NR 1997, 'The role of context on α-helix stabilization: Host-guest analysis in a mixed background peptide model', Protein Science, vol. 6, no. 6, pp. 1264-1272.
Yang, Jianxin ; Spek, Erik J. ; Gong, Youxiang ; Zhou, Hongxing ; Kallenbach, Neville R. / The role of context on α-helix stabilization : Host-guest analysis in a mixed background peptide model. In: Protein Science. 1997 ; Vol. 6, No. 6. pp. 1264-1272.
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