The Requirement of Nkx2-1 in the Temporal Specification of Cortical Interneuron Subtypes

Simon J.B. Butt, Vitor H. Sousa, Marc V. Fuccillo, Jens Hjerling-Leffler, Goichi Miyoshi, Shioko Kimura, Gordon Fishell

Research output: Contribution to journalArticle

Abstract

Previous work has demonstrated that the character of mouse cortical interneuron subtypes can be directly related to their embryonic temporal and spatial origins. The relationship between embryonic origin and the character of mature interneurons is likely reflected by the developmental expression of genes that direct cell fate. However, a thorough understanding of the early genetic events that specify subtype identity has been hampered by the perinatal lethality resulting from the loss of genes implicated in the determination of cortical interneurons. Here, we employ a conditional loss-of-function approach to demonstrate that the transcription factor Nkx2-1 is required for the proper specification of specific interneuron subtypes. Removal of this gene at distinct neurogenic time points results in a switch in the subtypes of neurons observed at more mature ages. Our strategy reveals a causal link between the embryonic genetic specification by Nkx2-1 in progenitors and the functional attributes of their neuronal progeny in the mature nervous system.

Original languageEnglish (US)
Pages (from-to)722-732
Number of pages11
JournalNeuron
Volume59
Issue number5
DOIs
StatePublished - Sep 11 2008

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Interneurons
Developmental Genes
Nervous System
Genes
Transcription Factors
Neurons

Keywords

  • DEVBIO
  • SIGNALING

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Butt, S. J. B., Sousa, V. H., Fuccillo, M. V., Hjerling-Leffler, J., Miyoshi, G., Kimura, S., & Fishell, G. (2008). The Requirement of Nkx2-1 in the Temporal Specification of Cortical Interneuron Subtypes. Neuron, 59(5), 722-732. https://doi.org/10.1016/j.neuron.2008.07.031

The Requirement of Nkx2-1 in the Temporal Specification of Cortical Interneuron Subtypes. / Butt, Simon J.B.; Sousa, Vitor H.; Fuccillo, Marc V.; Hjerling-Leffler, Jens; Miyoshi, Goichi; Kimura, Shioko; Fishell, Gordon.

In: Neuron, Vol. 59, No. 5, 11.09.2008, p. 722-732.

Research output: Contribution to journalArticle

Butt, SJB, Sousa, VH, Fuccillo, MV, Hjerling-Leffler, J, Miyoshi, G, Kimura, S & Fishell, G 2008, 'The Requirement of Nkx2-1 in the Temporal Specification of Cortical Interneuron Subtypes', Neuron, vol. 59, no. 5, pp. 722-732. https://doi.org/10.1016/j.neuron.2008.07.031
Butt SJB, Sousa VH, Fuccillo MV, Hjerling-Leffler J, Miyoshi G, Kimura S et al. The Requirement of Nkx2-1 in the Temporal Specification of Cortical Interneuron Subtypes. Neuron. 2008 Sep 11;59(5):722-732. https://doi.org/10.1016/j.neuron.2008.07.031
Butt, Simon J.B. ; Sousa, Vitor H. ; Fuccillo, Marc V. ; Hjerling-Leffler, Jens ; Miyoshi, Goichi ; Kimura, Shioko ; Fishell, Gordon. / The Requirement of Nkx2-1 in the Temporal Specification of Cortical Interneuron Subtypes. In: Neuron. 2008 ; Vol. 59, No. 5. pp. 722-732.
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