The regulation of forkhead/HNF-3β expression in the Ciona embryo

Anna Di Gregorio, Joseph C. Corbo, Michael Levine

Research output: Contribution to journalArticle

Abstract

The Ciona forkhead/HNF-3β gene (Ci-fkh) is expressed in the primary axial tissues of the developing tadpole, including the notochord, endoderm, and rudimentary floor plate of the CNS. In an effort to determine the basis for this complex pattern of expression we have conducted a detailed analysis of the Ci-fkh 5′-regulatory region. Different 5′ sequences were attached to a lacZ reporter gene and analyzed in electroporated Ciona embryos. A short regulatory sequence (AS) located ∼1.7 kb upstream of the transcribed region is shown to be essential for expression in all three axial tissues. The proximal 20 bp of the AS contains overlapping Snail repressor elements and a T-box motif. Deleting these sequences causes the loss of reporter gene expression in the endoderm, as well as expanded expression in the neural tube. These results suggest that a T-box gene such as Ci-VegTR activates Ci-fkh expression in the endoderm, while the Ci-Sna repressor excludes expression from the lateral ependymal cells and restricts the Ci-fkh pattern to the rudimentary floor plate in ventral regions of the neural tube. We also present evidence for Ci-fkh positive autofeedback, whereby the Ci-Fkh protein binds to critical activator sites within the Ci-fkh 5′-regulatory region and helps maintain high levels of expression. We discuss these results with respect to forkhead/HNF-3β regulation in vertebrates.

Original languageEnglish (US)
Pages (from-to)31-43
Number of pages13
JournalDevelopmental Biology
Volume229
Issue number1
DOIs
StatePublished - Jan 1 2001

Fingerprint

Embryonic Structures
Endoderm
Genes
Neural Tube
Nucleic Acid Regulatory Sequences
Reporter Genes
Notochord
Gene Expression
Lac Operon
Snails
Larva
Vertebrates
Proteins

Keywords

  • Ascidian
  • Ciona intestinalis
  • Endoderm
  • Forkhead
  • HNF-3β
  • Notochord
  • Spinal cord

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The regulation of forkhead/HNF-3β expression in the Ciona embryo. / Di Gregorio, Anna; Corbo, Joseph C.; Levine, Michael.

In: Developmental Biology, Vol. 229, No. 1, 01.01.2001, p. 31-43.

Research output: Contribution to journalArticle

Di Gregorio, Anna ; Corbo, Joseph C. ; Levine, Michael. / The regulation of forkhead/HNF-3β expression in the Ciona embryo. In: Developmental Biology. 2001 ; Vol. 229, No. 1. pp. 31-43.
@article{d53b86365645451590f5c5780f1ed291,
title = "The regulation of forkhead/HNF-3β expression in the Ciona embryo",
abstract = "The Ciona forkhead/HNF-3β gene (Ci-fkh) is expressed in the primary axial tissues of the developing tadpole, including the notochord, endoderm, and rudimentary floor plate of the CNS. In an effort to determine the basis for this complex pattern of expression we have conducted a detailed analysis of the Ci-fkh 5′-regulatory region. Different 5′ sequences were attached to a lacZ reporter gene and analyzed in electroporated Ciona embryos. A short regulatory sequence (AS) located ∼1.7 kb upstream of the transcribed region is shown to be essential for expression in all three axial tissues. The proximal 20 bp of the AS contains overlapping Snail repressor elements and a T-box motif. Deleting these sequences causes the loss of reporter gene expression in the endoderm, as well as expanded expression in the neural tube. These results suggest that a T-box gene such as Ci-VegTR activates Ci-fkh expression in the endoderm, while the Ci-Sna repressor excludes expression from the lateral ependymal cells and restricts the Ci-fkh pattern to the rudimentary floor plate in ventral regions of the neural tube. We also present evidence for Ci-fkh positive autofeedback, whereby the Ci-Fkh protein binds to critical activator sites within the Ci-fkh 5′-regulatory region and helps maintain high levels of expression. We discuss these results with respect to forkhead/HNF-3β regulation in vertebrates.",
keywords = "Ascidian, Ciona intestinalis, Endoderm, Forkhead, HNF-3β, Notochord, Spinal cord",
author = "{Di Gregorio}, Anna and Corbo, {Joseph C.} and Michael Levine",
year = "2001",
month = "1",
day = "1",
doi = "10.1006/dbio.2000.9964",
language = "English (US)",
volume = "229",
pages = "31--43",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The regulation of forkhead/HNF-3β expression in the Ciona embryo

AU - Di Gregorio, Anna

AU - Corbo, Joseph C.

AU - Levine, Michael

PY - 2001/1/1

Y1 - 2001/1/1

N2 - The Ciona forkhead/HNF-3β gene (Ci-fkh) is expressed in the primary axial tissues of the developing tadpole, including the notochord, endoderm, and rudimentary floor plate of the CNS. In an effort to determine the basis for this complex pattern of expression we have conducted a detailed analysis of the Ci-fkh 5′-regulatory region. Different 5′ sequences were attached to a lacZ reporter gene and analyzed in electroporated Ciona embryos. A short regulatory sequence (AS) located ∼1.7 kb upstream of the transcribed region is shown to be essential for expression in all three axial tissues. The proximal 20 bp of the AS contains overlapping Snail repressor elements and a T-box motif. Deleting these sequences causes the loss of reporter gene expression in the endoderm, as well as expanded expression in the neural tube. These results suggest that a T-box gene such as Ci-VegTR activates Ci-fkh expression in the endoderm, while the Ci-Sna repressor excludes expression from the lateral ependymal cells and restricts the Ci-fkh pattern to the rudimentary floor plate in ventral regions of the neural tube. We also present evidence for Ci-fkh positive autofeedback, whereby the Ci-Fkh protein binds to critical activator sites within the Ci-fkh 5′-regulatory region and helps maintain high levels of expression. We discuss these results with respect to forkhead/HNF-3β regulation in vertebrates.

AB - The Ciona forkhead/HNF-3β gene (Ci-fkh) is expressed in the primary axial tissues of the developing tadpole, including the notochord, endoderm, and rudimentary floor plate of the CNS. In an effort to determine the basis for this complex pattern of expression we have conducted a detailed analysis of the Ci-fkh 5′-regulatory region. Different 5′ sequences were attached to a lacZ reporter gene and analyzed in electroporated Ciona embryos. A short regulatory sequence (AS) located ∼1.7 kb upstream of the transcribed region is shown to be essential for expression in all three axial tissues. The proximal 20 bp of the AS contains overlapping Snail repressor elements and a T-box motif. Deleting these sequences causes the loss of reporter gene expression in the endoderm, as well as expanded expression in the neural tube. These results suggest that a T-box gene such as Ci-VegTR activates Ci-fkh expression in the endoderm, while the Ci-Sna repressor excludes expression from the lateral ependymal cells and restricts the Ci-fkh pattern to the rudimentary floor plate in ventral regions of the neural tube. We also present evidence for Ci-fkh positive autofeedback, whereby the Ci-Fkh protein binds to critical activator sites within the Ci-fkh 5′-regulatory region and helps maintain high levels of expression. We discuss these results with respect to forkhead/HNF-3β regulation in vertebrates.

KW - Ascidian

KW - Ciona intestinalis

KW - Endoderm

KW - Forkhead

KW - HNF-3β

KW - Notochord

KW - Spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0035166739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035166739&partnerID=8YFLogxK

U2 - 10.1006/dbio.2000.9964

DO - 10.1006/dbio.2000.9964

M3 - Article

VL - 229

SP - 31

EP - 43

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -