The processing of a Benzo(a)pyrene adduct into a frameshift or a base substitution mutation requires a different set of genes in Escherichia coli

Nathalie Lenne-Samuel, Regine Janel-Bintz, Aleksandr Kolbanovskiy, Nicholas E. Geacintov, Robert P P Fuchs

Research output: Contribution to journalArticle

Abstract

Replication through a single DNA lesion may give rise to a panel of translesion synthesis (TLS) events, which comprise error-free TLS, base substitutions and frameshift mutations. In order to determine the genetic control of the various TLS events induced by a single lesion, we have chosen the major N2-dG adduct of (+)-anti-Benzo(a)pyrene diol epoxide [(+)-anti-BPDE] adduct located within a short run of guanines as a model lesion. Within this sequence context, in addition to the major event, i.e. error-free TLS, the adduct also induces base substitutions (mostly G → T transversions) and -1 frameshift mutations. The pathway leading to G → T base substitution mutagenesis appears to be SOS independent, suggesting that TLS is most probably performed by the replicative Pol III holoenzyme itself. In contrast, both error-free and frameshift TLS pathways are dependent upon SOS-encoded functions that belong to the pool of inducible DNA polymerases specialized in TLS (translesional DNA polymerases), namely umuDC (Pol V) and dinB (Pol IV). It is likely that, given the diversity of conformations that can be adopted by lesion-containing replication intermediates, cells use one or several translesional DNA polymerases to achieve TLS.

Original languageEnglish (US)
Pages (from-to)299-307
Number of pages9
JournalMolecular Microbiology
Volume38
Issue number2
DOIs
StatePublished - 2000

Fingerprint

Benzo(a)pyrene
DNA-Directed DNA Polymerase
Frameshift Mutation
Escherichia coli
Mutation
Genes
Holoenzymes
Epoxy Compounds
Guanine
Mutagenesis
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The processing of a Benzo(a)pyrene adduct into a frameshift or a base substitution mutation requires a different set of genes in Escherichia coli. / Lenne-Samuel, Nathalie; Janel-Bintz, Regine; Kolbanovskiy, Aleksandr; Geacintov, Nicholas E.; Fuchs, Robert P P.

In: Molecular Microbiology, Vol. 38, No. 2, 2000, p. 299-307.

Research output: Contribution to journalArticle

Lenne-Samuel, Nathalie ; Janel-Bintz, Regine ; Kolbanovskiy, Aleksandr ; Geacintov, Nicholas E. ; Fuchs, Robert P P. / The processing of a Benzo(a)pyrene adduct into a frameshift or a base substitution mutation requires a different set of genes in Escherichia coli. In: Molecular Microbiology. 2000 ; Vol. 38, No. 2. pp. 299-307.
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