The plant hormone ethylene restricts Arabidopsis growth via the epidermis

Irina Ivanova Vaseva, Enas Qudeimat, Thomas Potuschak, Yunlong Du, Pascal Genschik, Filip Vandenbussche, Dominique Van Der Straeten

    Research output: Contribution to journalArticle

    Abstract

    The gaseous hormone ethylene plays a key role in plant growth and development, and it is a major regulator of stress responses. It inhibits vegetative growth by restricting cell elongation, mainly through cross-talk with auxins. However, it remains unknown whether ethylene controls growth throughout all plant tissues or whether its signaling is confined to specific cell types. We employed a targeted expression approach to map the tissue site(s) of ethylene growth regulation. The ubiquitin E3 ligase complex containing Skp1, Cullin1, and the F-box protein EBF1 or EBF2 (SCFEBF1/2) target the degradation of EIN3, the master transcription factor in ethylene signaling. We coupled EBF1 and EBF2 to a number of cell type-specific promoters. Using phenotypic assays for ethylene response and mutant complementation, we revealed that the epidermis is the main site of ethylene action controlling plant growth in both roots and shoots. Suppression of ethylene signaling in the epidermis of the constitutive ethylene signaling mutant ctr1-1 was sufficient to rescue the mutant phenotype, pointing to the epidermis as a key cell type required for ethylene-mediated growth inhibition.

    Original languageEnglish (US)
    Pages (from-to)E4130-E4139
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume115
    Issue number17
    DOIs
    StatePublished - Apr 24 2018

    Fingerprint

    Plant Growth Regulators
    Arabidopsis
    Epidermis
    Growth
    F-Box Proteins
    ethylene
    Indoleacetic Acids
    Ubiquitin-Protein Ligases
    Plant Development
    Growth and Development
    Transcription Factors
    Cell Count
    Hormones
    Phenotype

    Keywords

    • Arabidopsis
    • Auxin
    • EIN3 binding F-box factor EBF
    • Ethylene
    • Root/shoot

    ASJC Scopus subject areas

    • General

    Cite this

    Vaseva, I. I., Qudeimat, E., Potuschak, T., Du, Y., Genschik, P., Vandenbussche, F., & Van Der Straeten, D. (2018). The plant hormone ethylene restricts Arabidopsis growth via the epidermis. Proceedings of the National Academy of Sciences of the United States of America, 115(17), E4130-E4139. https://doi.org/10.1073/pnas.1717649115

    The plant hormone ethylene restricts Arabidopsis growth via the epidermis. / Vaseva, Irina Ivanova; Qudeimat, Enas; Potuschak, Thomas; Du, Yunlong; Genschik, Pascal; Vandenbussche, Filip; Van Der Straeten, Dominique.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 17, 24.04.2018, p. E4130-E4139.

    Research output: Contribution to journalArticle

    Vaseva, II, Qudeimat, E, Potuschak, T, Du, Y, Genschik, P, Vandenbussche, F & Van Der Straeten, D 2018, 'The plant hormone ethylene restricts Arabidopsis growth via the epidermis', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 17, pp. E4130-E4139. https://doi.org/10.1073/pnas.1717649115
    Vaseva, Irina Ivanova ; Qudeimat, Enas ; Potuschak, Thomas ; Du, Yunlong ; Genschik, Pascal ; Vandenbussche, Filip ; Van Der Straeten, Dominique. / The plant hormone ethylene restricts Arabidopsis growth via the epidermis. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 17. pp. E4130-E4139.
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