The physicochemical characterization and in vivo response of micro/nanoporous bioactive ceramic particulate bone graft materials

Nick Tovar, Ryo Jimbo, Lukasz Witek, Rodolfo Anchieta, Daniel Yoo, Lakshmipradha Manne, Lucas Machado, Riddhi Gangolli, Paulo Coelho

Research output: Contribution to journalArticle

Abstract

In this study, the physicochemical characteristics of calcium phosphate based bioactive ceramics of different compositions and blends presenting similar micro/nanoporosity and micrometer scale surface texture were characterized and evaluated in an in vivo model. Prior to the animal experiment, the porosity, surface area, particle size distribution, phase quantification, and dissolution of the materials tested were evaluated. The bone regenerative properties of the materials were evaluated using a rabbit calvaria model. After 2, 4, and 8 weeks, the animals were sacrificed and all samples were subjected to histologic observation and histomorphometric analysis. The material characterization showed that all materials tested presented variation in particle size, porosity and composition with different degrees of HA/TCP/lower stoichiometry phase ratios. Histologically, the calvarial defects presented temporal bone filling suggesting that all material groups were biocompatible and osteoconductive. Among the different materials tested, there were significant differences found in the amount of bone formation as a function of time. At 8 weeks, the micro/nanoporous material presenting ~ 55%TCP:45%HA composition ratio presented higher amounts of new bone regeneration relative to other blends and a decrease in the amount of soft tissue infiltration.

Original languageEnglish (US)
Pages (from-to)472-480
Number of pages9
JournalMaterials Science and Engineering C
Volume43
DOIs
StatePublished - Oct 1 2014

Fingerprint

Porosity
Ceramics
activity (biology)
Particle Size
Grafts
particulates
bones
Bone
ceramics
Transplants
Bone and Bones
Bone Regeneration
animals
Temporal Bone
Osteogenesis
Skull
osteogenesis
porosity
calcium phosphates
rabbits

Keywords

  • Animal experiment
  • Calcium phosphate materials
  • Histomorphometry
  • Porosity

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials
  • Medicine(all)

Cite this

The physicochemical characterization and in vivo response of micro/nanoporous bioactive ceramic particulate bone graft materials. / Tovar, Nick; Jimbo, Ryo; Witek, Lukasz; Anchieta, Rodolfo; Yoo, Daniel; Manne, Lakshmipradha; Machado, Lucas; Gangolli, Riddhi; Coelho, Paulo.

In: Materials Science and Engineering C, Vol. 43, 01.10.2014, p. 472-480.

Research output: Contribution to journalArticle

Tovar, Nick ; Jimbo, Ryo ; Witek, Lukasz ; Anchieta, Rodolfo ; Yoo, Daniel ; Manne, Lakshmipradha ; Machado, Lucas ; Gangolli, Riddhi ; Coelho, Paulo. / The physicochemical characterization and in vivo response of micro/nanoporous bioactive ceramic particulate bone graft materials. In: Materials Science and Engineering C. 2014 ; Vol. 43. pp. 472-480.
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