The origin and diversification of the merozoite surface protein 3 (msp3) multi-gene family in Plasmodium vivax and related parasites

Benjamin L. Rice, Mónica M. Acosta, M. Andreína Pacheco, Jane M. Carlton, John W. Barnwell, Ananias A. Escalante

Research output: Contribution to journalArticle

Abstract

The genus Plasmodium is a diversified group of parasites with more than 200 known species that includes those causing malaria in humans. These parasites use numerous proteins in a complex process that allows them to invade the red blood cells of their vertebrate hosts. Many of those proteins are part of multi-gene families; one of which is the merozoite surface protein-3 (msp3) family. The msp3 multi-gene family is considered important in the two main human parasites, Plasmodium vivax and Plasmodium falciparum, as its paralogs are simultaneously expressed in the blood stage (merozoite) and are immunogenic. There are large differences among Plasmodium species in the number of paralogs in this family. Such differences have been previously explained, in part, as adaptations that allow the different Plasmodium species to invade their hosts. To investigate this, we characterized the array containing msp3 genes among several Plasmodium species, including P. falciparum and P. vivax. We first found no evidence indicating that the msp3 family of P. falciparum was homologous to that of P. vivax. Subsequently, by focusing on the diverse clade of nonhuman primate parasites to which P. vivax is closely related, where homology was evident, we found no evidence indicating that the interspecies variation in the number of paralogs was an adaptation related to changes in host range or host switches. Overall, we hypothesize that the evolution of the msp3 family in P. vivax is consistent with a model of multi-allelic diversifying selection where the paralogs may have functionally redundant roles in terms of increasing antigenic diversity. Thus, we suggest that the expressed MSP3 proteins could serve as "decoys", via antigenic diversity, during the critical process of invading the host red blood cells.

Original languageEnglish (US)
Pages (from-to)172-184
Number of pages13
JournalMolecular Phylogenetics and Evolution
Volume78
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Plasmodium vivax
Merozoites
merozoites
surface proteins
multigene family
Membrane Proteins
parasite
Parasites
Plasmodium
parasites
protein
gene
Plasmodium falciparum
Genes
Antigenic Variation
antigenic variation
blood
erythrocytes
Erythrocytes
Proteins

Keywords

  • Homology
  • Merozoite surface protein 3
  • Multi-gene family
  • Plasmodium
  • Plasmodium falciparum
  • Plasmodium vivax

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

The origin and diversification of the merozoite surface protein 3 (msp3) multi-gene family in Plasmodium vivax and related parasites. / Rice, Benjamin L.; Acosta, Mónica M.; Pacheco, M. Andreína; Carlton, Jane M.; Barnwell, John W.; Escalante, Ananias A.

In: Molecular Phylogenetics and Evolution, Vol. 78, No. 1, 2014, p. 172-184.

Research output: Contribution to journalArticle

Rice, Benjamin L. ; Acosta, Mónica M. ; Pacheco, M. Andreína ; Carlton, Jane M. ; Barnwell, John W. ; Escalante, Ananias A. / The origin and diversification of the merozoite surface protein 3 (msp3) multi-gene family in Plasmodium vivax and related parasites. In: Molecular Phylogenetics and Evolution. 2014 ; Vol. 78, No. 1. pp. 172-184.
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