Abstract
The nature of the ligation of N-methylformamide (NMF) to the metal sites of FeMoco has been investigated. The IR spectrum of FeMoco in NMF shows an intense band at 1600 cm-1 which we assign to the v(CO) stretch of ligated NMF. This frequency is characteristic of N-deprotonated amide ligands, indicating that NMF ligation to FeMoco is predominantly through nitrogen. Hence NMF behaves as an anionic ligand. The importance of the amide functionality for FeMoco extraction has been further examined and a new extracting agent, 2-pyrrolidinone, has been employed. We hypothesize that, in the MoFe-protein, the primary coordination of the cofactor is via deprotonated backbone amide ligands.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 561-565 |
| Number of pages | 5 |
| Journal | Polyhedron |
| Volume | 5 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 1986 |
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ASJC Scopus subject areas
- Biochemistry
- Inorganic Chemistry
- Physical and Theoretical Chemistry
- Materials Chemistry
Cite this
The nature of amide ligation to the metal sites of FeMoco. / Walters, Marc; Chapman, Stephen K.; Orme-Johnson, William H.
In: Polyhedron, Vol. 5, No. 1-2, 1986, p. 561-565.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The nature of amide ligation to the metal sites of FeMoco
AU - Walters, Marc
AU - Chapman, Stephen K.
AU - Orme-Johnson, William H.
PY - 1986
Y1 - 1986
N2 - The nature of the ligation of N-methylformamide (NMF) to the metal sites of FeMoco has been investigated. The IR spectrum of FeMoco in NMF shows an intense band at 1600 cm-1 which we assign to the v(CO) stretch of ligated NMF. This frequency is characteristic of N-deprotonated amide ligands, indicating that NMF ligation to FeMoco is predominantly through nitrogen. Hence NMF behaves as an anionic ligand. The importance of the amide functionality for FeMoco extraction has been further examined and a new extracting agent, 2-pyrrolidinone, has been employed. We hypothesize that, in the MoFe-protein, the primary coordination of the cofactor is via deprotonated backbone amide ligands.
AB - The nature of the ligation of N-methylformamide (NMF) to the metal sites of FeMoco has been investigated. The IR spectrum of FeMoco in NMF shows an intense band at 1600 cm-1 which we assign to the v(CO) stretch of ligated NMF. This frequency is characteristic of N-deprotonated amide ligands, indicating that NMF ligation to FeMoco is predominantly through nitrogen. Hence NMF behaves as an anionic ligand. The importance of the amide functionality for FeMoco extraction has been further examined and a new extracting agent, 2-pyrrolidinone, has been employed. We hypothesize that, in the MoFe-protein, the primary coordination of the cofactor is via deprotonated backbone amide ligands.
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UR - http://www.scopus.com/inward/citedby.url?scp=0346852367&partnerID=8YFLogxK
U2 - 10.1016/S0277-5387(00)84964-5
DO - 10.1016/S0277-5387(00)84964-5
M3 - Article
VL - 5
SP - 561
EP - 565
JO - Polyhedron
JF - Polyhedron
SN - 0277-5387
IS - 1-2
ER -