The nature of amide ligation to the metal sites of FeMoco

Marc Walters, Stephen K. Chapman, William H. Orme-Johnson

Research output: Contribution to journalArticle

Abstract

The nature of the ligation of N-methylformamide (NMF) to the metal sites of FeMoco has been investigated. The IR spectrum of FeMoco in NMF shows an intense band at 1600 cm-1 which we assign to the v(CO) stretch of ligated NMF. This frequency is characteristic of N-deprotonated amide ligands, indicating that NMF ligation to FeMoco is predominantly through nitrogen. Hence NMF behaves as an anionic ligand. The importance of the amide functionality for FeMoco extraction has been further examined and a new extracting agent, 2-pyrrolidinone, has been employed. We hypothesize that, in the MoFe-protein, the primary coordination of the cofactor is via deprotonated backbone amide ligands.

Original languageEnglish (US)
Pages (from-to)561-565
Number of pages5
JournalPolyhedron
Volume5
Issue number1-2
DOIs
StatePublished - 1986

Fingerprint

methylformamide
Amides
amides
Ligation
Metals
Ligands
ligands
metals
Molybdoferredoxin
Nitrogen
proteins
Proteins
nitrogen
Carbon Monoxide

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry
  • Physical and Theoretical Chemistry
  • Materials Chemistry

Cite this

The nature of amide ligation to the metal sites of FeMoco. / Walters, Marc; Chapman, Stephen K.; Orme-Johnson, William H.

In: Polyhedron, Vol. 5, No. 1-2, 1986, p. 561-565.

Research output: Contribution to journalArticle

Walters, Marc ; Chapman, Stephen K. ; Orme-Johnson, William H. / The nature of amide ligation to the metal sites of FeMoco. In: Polyhedron. 1986 ; Vol. 5, No. 1-2. pp. 561-565.
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