The mRNA-bound proteome of the early fly embryo

Hans Hermann Wessels, Koshi Imami, Alexander G. Baltz, Marcin Kolinski, Anastasia Beldovskaya, Matthias Selbach, Stephen Small, Uwe Ohler, Markus Landthaler

Research output: Contribution to journalArticle

Abstract

Early embryogenesis is characterized by the maternal to zygotic transition (MZT), in which maternally deposited messenger RNAs are degraded while zygotic transcription begins. Before the MZT, post-transcriptional gene regulation by RNA-binding proteins (RBPs) is the dominant force in embryo patterning. We used two mRNA interactome capture methods to identify RBPs bound to polyadenylated transcripts within the first 2 h of Drosophila melanogaster embryogenesis. We identified a high-confidence set of 476 putative RBPs and confirmed RNA-binding activities for most of 24 tested candidates. Most proteins in the interactome are known RBPs or harbor canonical RBP features, but 99 exhibited previously uncharacterized RNA-binding activity. mRNA-bound RBPs and TFs exhibit distinct expression dynamics, in which the newly identified RBPs dominate the first 2 h of embryonic development. Integrating our resource with in situ hybridization data from existing databases showed that mRNAs encoding RBPs are enriched in posterior regions of the early embryo, suggesting their general importance in posterior patterning and germ cell maturation.

Original languageEnglish (US)
Pages (from-to)1000-1009
Number of pages10
JournalGenome Research
Volume26
Issue number7
DOIs
StatePublished - Jul 1 2016

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RNA-Binding Proteins
Proteome
Diptera
Embryonic Structures
Messenger RNA
Embryonic Development
Mothers
RNA
Drosophila melanogaster
Germ Cells
In Situ Hybridization
Databases

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Wessels, H. H., Imami, K., Baltz, A. G., Kolinski, M., Beldovskaya, A., Selbach, M., ... Landthaler, M. (2016). The mRNA-bound proteome of the early fly embryo. Genome Research, 26(7), 1000-1009. https://doi.org/10.1101/gr.200386.115

The mRNA-bound proteome of the early fly embryo. / Wessels, Hans Hermann; Imami, Koshi; Baltz, Alexander G.; Kolinski, Marcin; Beldovskaya, Anastasia; Selbach, Matthias; Small, Stephen; Ohler, Uwe; Landthaler, Markus.

In: Genome Research, Vol. 26, No. 7, 01.07.2016, p. 1000-1009.

Research output: Contribution to journalArticle

Wessels, HH, Imami, K, Baltz, AG, Kolinski, M, Beldovskaya, A, Selbach, M, Small, S, Ohler, U & Landthaler, M 2016, 'The mRNA-bound proteome of the early fly embryo', Genome Research, vol. 26, no. 7, pp. 1000-1009. https://doi.org/10.1101/gr.200386.115
Wessels HH, Imami K, Baltz AG, Kolinski M, Beldovskaya A, Selbach M et al. The mRNA-bound proteome of the early fly embryo. Genome Research. 2016 Jul 1;26(7):1000-1009. https://doi.org/10.1101/gr.200386.115
Wessels, Hans Hermann ; Imami, Koshi ; Baltz, Alexander G. ; Kolinski, Marcin ; Beldovskaya, Anastasia ; Selbach, Matthias ; Small, Stephen ; Ohler, Uwe ; Landthaler, Markus. / The mRNA-bound proteome of the early fly embryo. In: Genome Research. 2016 ; Vol. 26, No. 7. pp. 1000-1009.
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