The LIM homeodomain protein Lim-1 is widely expressed in neural, neural crest and mesoderm derivatives in vertebrate development

Alex A. Karavanov, Jean-Pierre Saint-Jeannet, Irina Karavanova, Masanori Taira, Igor B. Dawid

Research output: Contribution to journalArticle

Abstract

Polyclonal antibodies to Xlim-1 homeodomain protein of Xenopus laevis were used to study the developmental expression pattern of this protein in Xenopus, rat and mouse. Western blotting of embryo extracts injected with different Xlim-1 constructs confirmed the specificity of the antibody. Beginning at the gastrula stage, Xlim-1 protein was detected in three cell lineages: (i) notochord, (ii) pronephros and (iii) certain regions of the central nervous system, in agreement with earlier studies of the expression of Xlim-1 RNA. In addition, several new locations of Xlim-1 expression were found, including the olfactory organ, retina, otic vesicle, dorsal root ganglia and adrenal gland. Similar expression patterns were seen for the Lim-1 protein in frog and rodent tissues. These observations implicate the Xlim-1 gene in the specification of multiple cell lineages, particularly within the nervous system, and emphasize the conserved nature of the role of this gene in different vertebrate animals.

Original languageEnglish (US)
Pages (from-to)453-461
Number of pages9
JournalInternational Journal of Developmental Biology
Volume40
Issue number2
StatePublished - 1996

Fingerprint

LIM-Homeodomain Proteins
Neural Crest
Cell Lineage
Mesoderm
Vertebrates
Pronephros
Xenopus Proteins
Notochord
Homeodomain Proteins
Gastrula
Antibody Specificity
Xenopus laevis
Spinal Ganglia
Adrenal Glands
Anura
Nervous System
Genes
Ear
Retina
Rodentia

Keywords

  • Homeodomain
  • LIM domain
  • Lim-1 antibody
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

The LIM homeodomain protein Lim-1 is widely expressed in neural, neural crest and mesoderm derivatives in vertebrate development. / Karavanov, Alex A.; Saint-Jeannet, Jean-Pierre; Karavanova, Irina; Taira, Masanori; Dawid, Igor B.

In: International Journal of Developmental Biology, Vol. 40, No. 2, 1996, p. 453-461.

Research output: Contribution to journalArticle

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AU - Dawid, Igor B.

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