The Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) Study

Design and Methods for Complex DNA Analysis

Jacquelyn Taylor, Michelle L. Wright, Cindy A. Crusto, Yan V. Sun

Research output: Contribution to journalArticle

Abstract

The Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) study aims to delineate the independent and interaction effects of genomic (genetic and epigenetic) and psychological–environmental (maternally perceived racial discrimination, mental health, and parenting behavior) factors on blood pressure (BP) among African American mother–child dyads over time. The purpose of this article is to describe the two-step genetic and epigenetic approach that will be executed to explore Gene × Environment interactions on BP using a longitudinal cohort design. Procedure for the single collection of DNA at Time 1 includes the use of the Oragene 500-format saliva sample collection tube, which provides enough DNA for both the Illumina Multi-Ethnic Genotyping and 850K EPIC methylation analyses. BP readings, height, weight, percentage of body fat, and percentage of body water will be measured on all participants every 6 months for 2 years for a total of 4 time points. Genomic data analyses to be completed include multivariate modeling, assessment of population admixture and structure, and extended analyses including Bonferroni correction, false discovery rate methods, Monte Carlo approach, EIGENSTRAT methods, and so on, to determine relationships among both main and interaction effects of genetic, epigenetic, and psychological environmental factors on BP.

Original languageEnglish (US)
Pages (from-to)521-530
Number of pages10
JournalBiological Research for Nursing
Volume18
Issue number5
DOIs
StatePublished - Oct 1 2016

Fingerprint

Psychology
Blood Pressure
Epigenomics
DNA
Monte Carlo Method
Racism
Gene-Environment Interaction
Body Water
Parenting
Saliva
African Americans
Methylation
Adipose Tissue
Reading
Mental Health
Weights and Measures
Population

Keywords

  • African Americans
  • blood pressure
  • children
  • DNA methylation
  • gene–environment interaction
  • Infinium MethylationEPIC (850K) BeadChip
  • women

ASJC Scopus subject areas

  • Research and Theory

Cite this

The Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) Study : Design and Methods for Complex DNA Analysis. / Taylor, Jacquelyn; Wright, Michelle L.; Crusto, Cindy A.; Sun, Yan V.

In: Biological Research for Nursing, Vol. 18, No. 5, 01.10.2016, p. 521-530.

Research output: Contribution to journalArticle

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