The influence of terminal 3′,5′ phosphates on conformations of dApdA

S. Broyde, B. Hingerty

Research output: Contribution to journalArticle

Abstract

Addition of 3′ and 5′ terminal phosphates to dApdA causes a decrease in conformational flexibility. pdApdAp has much fewer conformers with energies below 2.5 kcal./mole than dApdA. The A, B and Watson-Crick (34) helices are the most preferred forms. Other important conformations are in the trans domain of ψ. Thus, flexibility in ψ, as well as in ω and ω, and in the sugar pucker is indicated. The transformation from the B helix to the Watson-Crick helix follows a low energy path. This is significant since Watson-Crick conformations may be important for intercalation into nucleic acid polymers (40-42) above the dimer level. The B helix is preferred over the A form in these larger DNA subunits.

Original languageEnglish (US)
Pages (from-to)2165-2178
Number of pages14
JournalNucleic Acids Research
Volume6
Issue number6
DOIs
StatePublished - 1979

Fingerprint

Helix
Phosphate
Conformation
Conformations
Phosphates
Nucleic acids
Intercalation
Sugars
Dimers
Nucleic Acids
Polymers
DNA
Flexibility
mole
Dimer
Energy
Decrease
Path
Influence
2'-deoxyadenylyl-(3'-5')-2'-deoxyadenosine

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

The influence of terminal 3′,5′ phosphates on conformations of dApdA. / Broyde, S.; Hingerty, B.

In: Nucleic Acids Research, Vol. 6, No. 6, 1979, p. 2165-2178.

Research output: Contribution to journalArticle

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