The influence of interleukin gene polymorphism on expression of interleukin-1β and tumor necrosis factor-α in periodontal tissue and gingival crevicular fluid

Steven Engebretson, Ira B. Lamster, Miriam Herrera-Abreu, Romanita S. Celenti, Janine M. Timms, Adeel G A Chaudhary, Francesco S. Di Giovine, Kenneth S. Kornman

Research output: Contribution to journalArticle

Abstract

Background: A specific composite genotype of the polymorphic interleukin-1 (IL-1) gene cluster has recently been associated with severe periodontitis. One polymorphism of the composite periodontitis-associated genotype (PAG) has been functionally linked with expression of high levels of IL-1. The purpose of this study was to test whether gingival crevicular fluid (GCF) levels of IL-1ß and tumor necrosis factor-alpha (TNFα), and gingival tissue levels of IL-1α, IL-1ß, and TNFα correlate with PAG, and to examine the effect of conservative periodontal therapy on these levels. Methods: Twenty-two adults with moderate to advanced periodontal disease were enrolled. Polymerase chain reaction amplification and restriction enzymes were used to identify specific polymorphisms from peripheral blood samples. GCF samples were collected at baseline and 3 weeks following conservative treatment and analyzed by ELISA for IL-1ß and TNFα. An interproximal gingival biopsy was collected at baseline and follow-up and analyzed for IL-1α, IL-1ß, and TNFα by ELISA. Results: The genotyping identified 7 as PAG(+) and 15 as PAG(-). The 2 groups were comparable in terms of existing periodontitis and age. In shallow sites (<4 mm), total IL-1ß in GCF was 2.5 times higher for PAG(+) patients prior to treatment (P = 0.03), and 2.2 times higher after treatment (P = 0.04), while differences were less apparent in deeper sites. Following treatment, a reduction in IL-1ß concentration in GCF was seen for PAG(-) but not for PAG(+) patients. While not statistically significant, a trend was observed in mean tissue levels of IL-1ß which were 3.6 times higher in PAG(+) versus PAG(-) patients (P = 0.09). Conclusions: These data suggest that PAG(+) patients may demonstrate phenotypic differences as indicated by elevated levels of IL-1ß in GCF.

Original languageEnglish (US)
Pages (from-to)567-573
Number of pages7
JournalJournal of Periodontology
Volume70
Issue number6
DOIs
StatePublished - Jun 1999

Fingerprint

Gingival Crevicular Fluid
Periodontitis
Interleukins
Interleukin-1
Tumor Necrosis Factor-alpha
Genotype
Genes
Enzyme-Linked Immunosorbent Assay
Periodontal Diseases
Multigene Family

Keywords

  • Cytokines
  • Gingival crevicular fluid
  • Interleukin-1
  • Periodontitis/therapy
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The influence of interleukin gene polymorphism on expression of interleukin-1β and tumor necrosis factor-α in periodontal tissue and gingival crevicular fluid. / Engebretson, Steven; Lamster, Ira B.; Herrera-Abreu, Miriam; Celenti, Romanita S.; Timms, Janine M.; Chaudhary, Adeel G A; Di Giovine, Francesco S.; Kornman, Kenneth S.

In: Journal of Periodontology, Vol. 70, No. 6, 06.1999, p. 567-573.

Research output: Contribution to journalArticle

Engebretson, Steven ; Lamster, Ira B. ; Herrera-Abreu, Miriam ; Celenti, Romanita S. ; Timms, Janine M. ; Chaudhary, Adeel G A ; Di Giovine, Francesco S. ; Kornman, Kenneth S. / The influence of interleukin gene polymorphism on expression of interleukin-1β and tumor necrosis factor-α in periodontal tissue and gingival crevicular fluid. In: Journal of Periodontology. 1999 ; Vol. 70, No. 6. pp. 567-573.
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abstract = "Background: A specific composite genotype of the polymorphic interleukin-1 (IL-1) gene cluster has recently been associated with severe periodontitis. One polymorphism of the composite periodontitis-associated genotype (PAG) has been functionally linked with expression of high levels of IL-1. The purpose of this study was to test whether gingival crevicular fluid (GCF) levels of IL-1{\ss} and tumor necrosis factor-alpha (TNFα), and gingival tissue levels of IL-1α, IL-1{\ss}, and TNFα correlate with PAG, and to examine the effect of conservative periodontal therapy on these levels. Methods: Twenty-two adults with moderate to advanced periodontal disease were enrolled. Polymerase chain reaction amplification and restriction enzymes were used to identify specific polymorphisms from peripheral blood samples. GCF samples were collected at baseline and 3 weeks following conservative treatment and analyzed by ELISA for IL-1{\ss} and TNFα. An interproximal gingival biopsy was collected at baseline and follow-up and analyzed for IL-1α, IL-1{\ss}, and TNFα by ELISA. Results: The genotyping identified 7 as PAG(+) and 15 as PAG(-). The 2 groups were comparable in terms of existing periodontitis and age. In shallow sites (<4 mm), total IL-1{\ss} in GCF was 2.5 times higher for PAG(+) patients prior to treatment (P = 0.03), and 2.2 times higher after treatment (P = 0.04), while differences were less apparent in deeper sites. Following treatment, a reduction in IL-1{\ss} concentration in GCF was seen for PAG(-) but not for PAG(+) patients. While not statistically significant, a trend was observed in mean tissue levels of IL-1{\ss} which were 3.6 times higher in PAG(+) versus PAG(-) patients (P = 0.09). Conclusions: These data suggest that PAG(+) patients may demonstrate phenotypic differences as indicated by elevated levels of IL-1{\ss} in GCF.",
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author = "Steven Engebretson and Lamster, {Ira B.} and Miriam Herrera-Abreu and Celenti, {Romanita S.} and Timms, {Janine M.} and Chaudhary, {Adeel G A} and {Di Giovine}, {Francesco S.} and Kornman, {Kenneth S.}",
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T1 - The influence of interleukin gene polymorphism on expression of interleukin-1β and tumor necrosis factor-α in periodontal tissue and gingival crevicular fluid

AU - Engebretson, Steven

AU - Lamster, Ira B.

AU - Herrera-Abreu, Miriam

AU - Celenti, Romanita S.

AU - Timms, Janine M.

AU - Chaudhary, Adeel G A

AU - Di Giovine, Francesco S.

AU - Kornman, Kenneth S.

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N2 - Background: A specific composite genotype of the polymorphic interleukin-1 (IL-1) gene cluster has recently been associated with severe periodontitis. One polymorphism of the composite periodontitis-associated genotype (PAG) has been functionally linked with expression of high levels of IL-1. The purpose of this study was to test whether gingival crevicular fluid (GCF) levels of IL-1ß and tumor necrosis factor-alpha (TNFα), and gingival tissue levels of IL-1α, IL-1ß, and TNFα correlate with PAG, and to examine the effect of conservative periodontal therapy on these levels. Methods: Twenty-two adults with moderate to advanced periodontal disease were enrolled. Polymerase chain reaction amplification and restriction enzymes were used to identify specific polymorphisms from peripheral blood samples. GCF samples were collected at baseline and 3 weeks following conservative treatment and analyzed by ELISA for IL-1ß and TNFα. An interproximal gingival biopsy was collected at baseline and follow-up and analyzed for IL-1α, IL-1ß, and TNFα by ELISA. Results: The genotyping identified 7 as PAG(+) and 15 as PAG(-). The 2 groups were comparable in terms of existing periodontitis and age. In shallow sites (<4 mm), total IL-1ß in GCF was 2.5 times higher for PAG(+) patients prior to treatment (P = 0.03), and 2.2 times higher after treatment (P = 0.04), while differences were less apparent in deeper sites. Following treatment, a reduction in IL-1ß concentration in GCF was seen for PAG(-) but not for PAG(+) patients. While not statistically significant, a trend was observed in mean tissue levels of IL-1ß which were 3.6 times higher in PAG(+) versus PAG(-) patients (P = 0.09). Conclusions: These data suggest that PAG(+) patients may demonstrate phenotypic differences as indicated by elevated levels of IL-1ß in GCF.

AB - Background: A specific composite genotype of the polymorphic interleukin-1 (IL-1) gene cluster has recently been associated with severe periodontitis. One polymorphism of the composite periodontitis-associated genotype (PAG) has been functionally linked with expression of high levels of IL-1. The purpose of this study was to test whether gingival crevicular fluid (GCF) levels of IL-1ß and tumor necrosis factor-alpha (TNFα), and gingival tissue levels of IL-1α, IL-1ß, and TNFα correlate with PAG, and to examine the effect of conservative periodontal therapy on these levels. Methods: Twenty-two adults with moderate to advanced periodontal disease were enrolled. Polymerase chain reaction amplification and restriction enzymes were used to identify specific polymorphisms from peripheral blood samples. GCF samples were collected at baseline and 3 weeks following conservative treatment and analyzed by ELISA for IL-1ß and TNFα. An interproximal gingival biopsy was collected at baseline and follow-up and analyzed for IL-1α, IL-1ß, and TNFα by ELISA. Results: The genotyping identified 7 as PAG(+) and 15 as PAG(-). The 2 groups were comparable in terms of existing periodontitis and age. In shallow sites (<4 mm), total IL-1ß in GCF was 2.5 times higher for PAG(+) patients prior to treatment (P = 0.03), and 2.2 times higher after treatment (P = 0.04), while differences were less apparent in deeper sites. Following treatment, a reduction in IL-1ß concentration in GCF was seen for PAG(-) but not for PAG(+) patients. While not statistically significant, a trend was observed in mean tissue levels of IL-1ß which were 3.6 times higher in PAG(+) versus PAG(-) patients (P = 0.09). Conclusions: These data suggest that PAG(+) patients may demonstrate phenotypic differences as indicated by elevated levels of IL-1ß in GCF.

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