The influence of different implant materials on human gingival fibroblast morphology, proliferation, and gene expression.

Seiichi Yamano, Andrew Kwok Yui Ma, Rabie M. Shanti, Soo Woo Kim, Keisuke Wada, Cortino Sukotjo

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate the cellular response of human gingival fibroblasts (HGFs) cultured on smooth or rough zirconia (Zr) or titanium (Ti) disks. Disks fabricated from four different materials--smooth Zr (Zr-S), rough Zr (Zr-R), smooth Ti (Ti-S), and rough Ti (Ti-R)--were used, and surface roughness was analyzed by atomic force microscopy. After HGFs were cultured on these disks, cell morphology was examined by scanning electron microscopy, cell proliferation activity was evaluated by a monotetrazolium assay, and gene expression levels of various collagens and integrins were measured by real-time polymerase chain reaction. The Ti-R disks were the roughest, followed by Zr-R, Ti-S, and Zr-S, in that order. The cells cultured on the Zr-S and Ti-S disks appeared to be more aligned with the fine irregularities at later time points, whereas the cells cultured on the Zr-S showed the weakest spreading compared to the other surfaces after 3 hours of culture. With respect to proliferation, cells proliferated significantly faster on the Zr-S surface than on the other surfaces. Gene expression of integrin α2 at 3 hours and integrin α5 and type I collagen at 48 hours on Zr-S was significantly up-regulated compared to Ti. Conversely, the expression of integrins β1 and β3 and type III collagen was significantly decreased on Zr-S at 1 hour compared to the other materials. These data indicate that different surface materials and topographies may induce a distinct HGF morphology, proliferation, and gene expression.

Original languageEnglish (US)
Pages (from-to)1247-1255
Number of pages9
JournalThe International journal of oral & maxillofacial implants
Volume26
Issue number6
StatePublished - Nov 2011

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Titanium
Fibroblasts
Gene Expression
Integrins
Cultured Cells
zirconium oxide
Cell Proliferation
Collagen Type V
Collagen Type III
Atomic Force Microscopy
Electron Scanning Microscopy
Real-Time Polymerase Chain Reaction
Collagen

ASJC Scopus subject areas

  • Medicine(all)

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The influence of different implant materials on human gingival fibroblast morphology, proliferation, and gene expression. / Yamano, Seiichi; Ma, Andrew Kwok Yui; Shanti, Rabie M.; Kim, Soo Woo; Wada, Keisuke; Sukotjo, Cortino.

In: The International journal of oral & maxillofacial implants, Vol. 26, No. 6, 11.2011, p. 1247-1255.

Research output: Contribution to journalArticle

Yamano, Seiichi ; Ma, Andrew Kwok Yui ; Shanti, Rabie M. ; Kim, Soo Woo ; Wada, Keisuke ; Sukotjo, Cortino. / The influence of different implant materials on human gingival fibroblast morphology, proliferation, and gene expression. In: The International journal of oral & maxillofacial implants. 2011 ; Vol. 26, No. 6. pp. 1247-1255.
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