The influence of diabetes on gingival crevicular fluid β-glucuronidase and interleukin-8

Steven Engebretson, Faranak Vossughi, Judith Hey-Hadavi, Gülnur Emingil, John T. Grbic

Research output: Contribution to journalArticle

Abstract

Objectives: Polymorphonuclear neutrophil (PMN) dysfunction is associated with diabetes. We examined the gingival crevicular fluid (GCF) β-glucuronidase (BG) and interleukin-8 (IL-8) levels of periodontitis patients with and without type 2 diabetes mellitus (DM). Material and methods: Forty five adults with type 2 DM and 32 adults without DM, both with chronic periodontitis were enrolled. GCF was collected from eight posterior sites in each quadrant, and periodontal parameters were recorded. GCF was assayed for IL-8 by ELISA and BG by a flourometric assay. Results: GCF IL-8 was positively correlated with probing depth (PD), and GCF BG but not clinical attachment level (CAL), bleeding on probing (BOP), or plaque index (PI). In contrast, GCF BG was strongly correlated with each of the clinical measures of periodontal disease. Subjects with DM significantly lower levels of both BG (73.0±44.8 versus 121.9±84.6 pg/sample; p=0.002) and IL-8 (32.1±33.1 versus 90.8±83.2 pg/sample; p<0.0001) even after adjustments for age, gender, PD, CAL, BOP, and PI. Neither BG nor IL-8 was correlated with HbA1c levels in subjects with DM. Conclusion: These data suggest that an inadequate local response by PMN, partially explained by an altered chemokine gradient, may contribute to periodontal disease in patients with type 2 DM.

Original languageEnglish (US)
Pages (from-to)784-790
Number of pages7
JournalJournal of Clinical Periodontology
Volume33
Issue number11
DOIs
StatePublished - Nov 2006

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Gingival Crevicular Fluid
Glucuronidase
Interleukin-8
Type 2 Diabetes Mellitus
Diabetes Mellitus
Periodontal Diseases
Neutrophils
Hemorrhage
Chronic Periodontitis
Periodontitis
Chemokines
Enzyme-Linked Immunosorbent Assay

Keywords

  • β-glucuronidase
  • Adult
  • Diabetes mellitus
  • Gingival crevicular fluid
  • HbA1c
  • Interleukin-8
  • Periodontitis
  • PMN

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The influence of diabetes on gingival crevicular fluid β-glucuronidase and interleukin-8. / Engebretson, Steven; Vossughi, Faranak; Hey-Hadavi, Judith; Emingil, Gülnur; Grbic, John T.

In: Journal of Clinical Periodontology, Vol. 33, No. 11, 11.2006, p. 784-790.

Research output: Contribution to journalArticle

Engebretson, Steven ; Vossughi, Faranak ; Hey-Hadavi, Judith ; Emingil, Gülnur ; Grbic, John T. / The influence of diabetes on gingival crevicular fluid β-glucuronidase and interleukin-8. In: Journal of Clinical Periodontology. 2006 ; Vol. 33, No. 11. pp. 784-790.
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abstract = "Objectives: Polymorphonuclear neutrophil (PMN) dysfunction is associated with diabetes. We examined the gingival crevicular fluid (GCF) β-glucuronidase (BG) and interleukin-8 (IL-8) levels of periodontitis patients with and without type 2 diabetes mellitus (DM). Material and methods: Forty five adults with type 2 DM and 32 adults without DM, both with chronic periodontitis were enrolled. GCF was collected from eight posterior sites in each quadrant, and periodontal parameters were recorded. GCF was assayed for IL-8 by ELISA and BG by a flourometric assay. Results: GCF IL-8 was positively correlated with probing depth (PD), and GCF BG but not clinical attachment level (CAL), bleeding on probing (BOP), or plaque index (PI). In contrast, GCF BG was strongly correlated with each of the clinical measures of periodontal disease. Subjects with DM significantly lower levels of both BG (73.0±44.8 versus 121.9±84.6 pg/sample; p=0.002) and IL-8 (32.1±33.1 versus 90.8±83.2 pg/sample; p<0.0001) even after adjustments for age, gender, PD, CAL, BOP, and PI. Neither BG nor IL-8 was correlated with HbA1c levels in subjects with DM. Conclusion: These data suggest that an inadequate local response by PMN, partially explained by an altered chemokine gradient, may contribute to periodontal disease in patients with type 2 DM.",
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