The influence of a novel propolis on mutans streptococci biofilms and caries development in rats

Simone Duarte, Pedro L. Rosalen, Mitsue F. Hayacibara, Jaime A. Cury, William H. Bowen, R. E. Marquis, V. L G Rehder, Adilson Sartoratto, Masaharu Ikegaki, Hyun Koo

Research output: Contribution to journalArticle

Abstract

A flavonoids-free Brazilian propolis (type 6) showed biological effects against mutans streptococci and inhibited the activity of glucosyltransferases. This study evaluated the influence of the ethanolic extract of a novel type of propolis (EEP) and its purified hexane fraction (EEH) on mutans streptococci biofilms and the development of dental caries in rats. The chemical composition of the propolis extracts were examined by gas chromatography/mass spectrometry. The effects of EEP and EEH on Streptococcus mutans UA159 and Streptococcus sobrinus 6715 biofilms were analysed by time-kill and glycolytic pH drop assays. Their influence on proton-translocating F-ATPase activity was also tested. In the animal study, the rats were infected with S. sobrinus 6715 and fed with cariogenic diet 2000. The rats were treated topically twice a day with each of the extracts (or control) for 5 weeks. After the experimental period, the microbial composition of their dental plaque and their caries scores were determined. The results showed that fatty acids (oleic, palmitic, linoleic and stearic) were the main compounds identified in EEP and EEH. These extracts did not show major effects on the viability of mutans streptococci biofilms. However, EEP and EEH significantly reduced acid production by the biofilms and also inhibited the activity of F-ATPase (60-65%). Furthermore, both extracts significantly reduced the incidence of smooth surface caries in vivo without displaying a reduction of the percentage of S. sobriuns in the animals' plaque (P < 0.05). However, only EEH was able to reduce the incidence and severity of sulcal surface caries (P < 0.05). The data suggest that the cariostatic properties of propolis type 6 are related to its effect on acid production and acid tolerance of cariogenic streptococci; the biological activities may be attributed to its high content of fatty acids.

Original languageEnglish (US)
Pages (from-to)15-22
Number of pages8
JournalArchives of Oral Biology
Volume51
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Propolis
Streptococcus mutans
Biofilms
Streptococcus sobrinus
Acids
Cariogenic Diet
Fatty Acids
Glucosyltransferases
Dental Plaque
Proton-Translocating ATPases
Incidence
Dental Caries
Hexanes
Streptococcus
Flavonoids
Gas Chromatography-Mass Spectrometry
Adenosine Triphosphatases

Keywords

  • Dental biofilm
  • Dental caries
  • Propolis
  • Virulence factors

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Duarte, S., Rosalen, P. L., Hayacibara, M. F., Cury, J. A., Bowen, W. H., Marquis, R. E., ... Koo, H. (2006). The influence of a novel propolis on mutans streptococci biofilms and caries development in rats. Archives of Oral Biology, 51(1), 15-22. https://doi.org/10.1016/j.archoralbio.2005.06.002

The influence of a novel propolis on mutans streptococci biofilms and caries development in rats. / Duarte, Simone; Rosalen, Pedro L.; Hayacibara, Mitsue F.; Cury, Jaime A.; Bowen, William H.; Marquis, R. E.; Rehder, V. L G; Sartoratto, Adilson; Ikegaki, Masaharu; Koo, Hyun.

In: Archives of Oral Biology, Vol. 51, No. 1, 01.2006, p. 15-22.

Research output: Contribution to journalArticle

Duarte, S, Rosalen, PL, Hayacibara, MF, Cury, JA, Bowen, WH, Marquis, RE, Rehder, VLG, Sartoratto, A, Ikegaki, M & Koo, H 2006, 'The influence of a novel propolis on mutans streptococci biofilms and caries development in rats', Archives of Oral Biology, vol. 51, no. 1, pp. 15-22. https://doi.org/10.1016/j.archoralbio.2005.06.002
Duarte, Simone ; Rosalen, Pedro L. ; Hayacibara, Mitsue F. ; Cury, Jaime A. ; Bowen, William H. ; Marquis, R. E. ; Rehder, V. L G ; Sartoratto, Adilson ; Ikegaki, Masaharu ; Koo, Hyun. / The influence of a novel propolis on mutans streptococci biofilms and caries development in rats. In: Archives of Oral Biology. 2006 ; Vol. 51, No. 1. pp. 15-22.
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