The graded distribution of the dorsal morphogen is initiated by selective nuclear transport in Drosophila

Christine Rushlow, Kyuhyung Han, James L. Manley, Michael Levine

Research output: Contribution to journalArticle

Abstract

The maternal morphogen dorsal (dl) plays a key role in the establishment of dorsal-ventral polarity in Drosophila. We present evidence that the graded distribution of dl protein is initiated by selective nuclear transport. The dl protein is uniformly distributed throughout the cytoplasm of early embryos, but approximately 90 min after fertilization, dl protein present in ventral but not dorsal regions is selectively transported to the nucleus. Mutations in maternally active genes that regulate dl disrupt this transport process, resulting in an inactive, cytoplasmically localized form of the dl protein. Selective nuclear transport of dl protein was reproduced in tissue culture cells. The wild-type dl protein is largely restricted to the cytoplasm, while truncated proteins are predominantly localized within the nucleus. Transient cotransfection assays suggest that dl activates expression from several promoters in an apparently sequence-independent manner. We discuss the role of nuclear transport as a regulated process in gene expression and development.

Original languageEnglish (US)
Pages (from-to)1165-1177
Number of pages13
JournalCell
Volume59
Issue number6
DOIs
StatePublished - Dec 22 1989

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Cell Nucleus Active Transport
Drosophila
Proteins
Cytoplasm
Tissue culture
Nuclear Proteins
Fertilization
Gene expression
Carrier Proteins
Embryonic Structures
Cell Culture Techniques
Mothers
Assays
Genes
Gene Expression
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The graded distribution of the dorsal morphogen is initiated by selective nuclear transport in Drosophila. / Rushlow, Christine; Han, Kyuhyung; Manley, James L.; Levine, Michael.

In: Cell, Vol. 59, No. 6, 22.12.1989, p. 1165-1177.

Research output: Contribution to journalArticle

Rushlow, Christine ; Han, Kyuhyung ; Manley, James L. ; Levine, Michael. / The graded distribution of the dorsal morphogen is initiated by selective nuclear transport in Drosophila. In: Cell. 1989 ; Vol. 59, No. 6. pp. 1165-1177.
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