The genome of melon (Cucumis melo L.)

Jordi Garcia-Mas, Andrej Benjak, Walter Sanseverino, Michael Bourgeois, Gisela Mir, Víctor M. Gonźalez, Elizabeth Henaff, Francisco Cam̂ara, Luca Cozzuto, Ernesto Lowy, Tyler Alioto, Salvador Capella-Gutieŕrez, Jose Blancae, Joaquín Cañizares, Pello Ziarsolo, Daniel Gonzalez-Ibeas, Luis Rodriǵuez-Moreno, Marcus Droege, Lei Du, Miguel Alvarez-TejadoBelen Lorente-Galdos, Marta Meleć, Luming Yang, Yiqun Weng, Arcadi Navarro, Tomas Marques-Bonet, Miguel A. Aranda, Fernando Nuez, Belén Picó, Toni Gabaldoń, Guglielmo Roma, Roderic Guigoć, Josep M. Casacuberta, Pere Aruś, Pere Puigdomeǹech

Research output: Contribution to journalArticle

Abstract

We report the genome sequence of melon, an important horticultural crop worldwide. We assembled 375 Mb of the double-haploid line DHL92, representing 83.3% of the estimatedmelon genome.We predicted 27,427 protein-coding genes, which we analyzed by reconstructing 22,218 phylogenetic trees, allowing mapping of the orthology and paralogy relationships of sequenced plant genomes. We observed the absence of recent whole-genome duplications in the melon lineage since the ancient eudicot triplication, and our data suggest that transposon amplification may in part explain the increased size of the melon genome compared with the close relative cucumber. A low number of nucleotide-binding site-leucine-rich repeat disease resistance genes were annotated, suggesting the existence of specific defense mechanisms in this species. The DHL92 genome was compared with that of its parental lines allowing the quantification of sequence variability in the species. The use of the genome sequence in future investigations will facilitate the understanding of evolution of cucurbits and the improvement of breeding strategies.

Original languageEnglish (US)
Pages (from-to)11872-11877
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number29
DOIs
StatePublished - Jul 17 2012

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Cucumis melo
Cucurbitaceae
Genome
Plant Genome
Genome Size
Cucumis sativus
Disease Resistance
Haploidy
Leucine
Breeding
Nucleotides
Binding Sites
Genes
Proteins

Keywords

  • De novo genome sequence
  • Phylome

ASJC Scopus subject areas

  • General

Cite this

Garcia-Mas, J., Benjak, A., Sanseverino, W., Bourgeois, M., Mir, G., Gonźalez, V. M., ... Puigdomeǹech, P. (2012). The genome of melon (Cucumis melo L.). Proceedings of the National Academy of Sciences of the United States of America, 109(29), 11872-11877. https://doi.org/10.1073/pnas.1205415109

The genome of melon (Cucumis melo L.). / Garcia-Mas, Jordi; Benjak, Andrej; Sanseverino, Walter; Bourgeois, Michael; Mir, Gisela; Gonźalez, Víctor M.; Henaff, Elizabeth; Cam̂ara, Francisco; Cozzuto, Luca; Lowy, Ernesto; Alioto, Tyler; Capella-Gutieŕrez, Salvador; Blancae, Jose; Cañizares, Joaquín; Ziarsolo, Pello; Gonzalez-Ibeas, Daniel; Rodriǵuez-Moreno, Luis; Droege, Marcus; Du, Lei; Alvarez-Tejado, Miguel; Lorente-Galdos, Belen; Meleć, Marta; Yang, Luming; Weng, Yiqun; Navarro, Arcadi; Marques-Bonet, Tomas; Aranda, Miguel A.; Nuez, Fernando; Picó, Belén; Gabaldoń, Toni; Roma, Guglielmo; Guigoć, Roderic; Casacuberta, Josep M.; Aruś, Pere; Puigdomeǹech, Pere.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 29, 17.07.2012, p. 11872-11877.

Research output: Contribution to journalArticle

Garcia-Mas, J, Benjak, A, Sanseverino, W, Bourgeois, M, Mir, G, Gonźalez, VM, Henaff, E, Cam̂ara, F, Cozzuto, L, Lowy, E, Alioto, T, Capella-Gutieŕrez, S, Blancae, J, Cañizares, J, Ziarsolo, P, Gonzalez-Ibeas, D, Rodriǵuez-Moreno, L, Droege, M, Du, L, Alvarez-Tejado, M, Lorente-Galdos, B, Meleć, M, Yang, L, Weng, Y, Navarro, A, Marques-Bonet, T, Aranda, MA, Nuez, F, Picó, B, Gabaldoń, T, Roma, G, Guigoć, R, Casacuberta, JM, Aruś, P & Puigdomeǹech, P 2012, 'The genome of melon (Cucumis melo L.)', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 29, pp. 11872-11877. https://doi.org/10.1073/pnas.1205415109
Garcia-Mas J, Benjak A, Sanseverino W, Bourgeois M, Mir G, Gonźalez VM et al. The genome of melon (Cucumis melo L.). Proceedings of the National Academy of Sciences of the United States of America. 2012 Jul 17;109(29):11872-11877. https://doi.org/10.1073/pnas.1205415109
Garcia-Mas, Jordi ; Benjak, Andrej ; Sanseverino, Walter ; Bourgeois, Michael ; Mir, Gisela ; Gonźalez, Víctor M. ; Henaff, Elizabeth ; Cam̂ara, Francisco ; Cozzuto, Luca ; Lowy, Ernesto ; Alioto, Tyler ; Capella-Gutieŕrez, Salvador ; Blancae, Jose ; Cañizares, Joaquín ; Ziarsolo, Pello ; Gonzalez-Ibeas, Daniel ; Rodriǵuez-Moreno, Luis ; Droege, Marcus ; Du, Lei ; Alvarez-Tejado, Miguel ; Lorente-Galdos, Belen ; Meleć, Marta ; Yang, Luming ; Weng, Yiqun ; Navarro, Arcadi ; Marques-Bonet, Tomas ; Aranda, Miguel A. ; Nuez, Fernando ; Picó, Belén ; Gabaldoń, Toni ; Roma, Guglielmo ; Guigoć, Roderic ; Casacuberta, Josep M. ; Aruś, Pere ; Puigdomeǹech, Pere. / The genome of melon (Cucumis melo L.). In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 29. pp. 11872-11877.
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abstract = "We report the genome sequence of melon, an important horticultural crop worldwide. We assembled 375 Mb of the double-haploid line DHL92, representing 83.3{\%} of the estimatedmelon genome.We predicted 27,427 protein-coding genes, which we analyzed by reconstructing 22,218 phylogenetic trees, allowing mapping of the orthology and paralogy relationships of sequenced plant genomes. We observed the absence of recent whole-genome duplications in the melon lineage since the ancient eudicot triplication, and our data suggest that transposon amplification may in part explain the increased size of the melon genome compared with the close relative cucumber. A low number of nucleotide-binding site-leucine-rich repeat disease resistance genes were annotated, suggesting the existence of specific defense mechanisms in this species. The DHL92 genome was compared with that of its parental lines allowing the quantification of sequence variability in the species. The use of the genome sequence in future investigations will facilitate the understanding of evolution of cucurbits and the improvement of breeding strategies.",
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AU - Gonźalez, Víctor M.

AU - Henaff, Elizabeth

AU - Cam̂ara, Francisco

AU - Cozzuto, Luca

AU - Lowy, Ernesto

AU - Alioto, Tyler

AU - Capella-Gutieŕrez, Salvador

AU - Blancae, Jose

AU - Cañizares, Joaquín

AU - Ziarsolo, Pello

AU - Gonzalez-Ibeas, Daniel

AU - Rodriǵuez-Moreno, Luis

AU - Droege, Marcus

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AU - Weng, Yiqun

AU - Navarro, Arcadi

AU - Marques-Bonet, Tomas

AU - Aranda, Miguel A.

AU - Nuez, Fernando

AU - Picó, Belén

AU - Gabaldoń, Toni

AU - Roma, Guglielmo

AU - Guigoć, Roderic

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AU - Puigdomeǹech, Pere

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