The genome and phenome of the green alga chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

David Nelson, Basel Khraiwesh, Weiqi Fu, Saleh Alseekh, Ashish Jaiswal, Amphun Chaiboonchoe, Khaled M. Hazzouri, Matthew O'Connor, Glenn Butterfoss, Nizar Drou, Jillian D. Rowe, Jamil Harb, Alisdair R. Fernie, Kristin Gunsalus, Kourosh Salehi-Ashtiani

Research output: Contribution to journalArticle

Abstract

To investigate the phenomic and genomic traits that allow green algae to survive in deserts, we characterized a ubiquitous species, Chloroidium sp. UTEX 3007, which we isolated from multiple locations in the United Arab Emirates (UAE). Metabolomic analyses of Chloroidium sp. UTEX 3007 indicated that the alga accumulates a broad range of carbon sources, including several desiccation tolerance-promoting sugars and unusually large stores of palmitate. Growth assays revealed capacities to grow in salinities from zero to 60 g/L and to grow heterotrophically on >40 distinct carbon sources. Assembly and annotation of genomic reads yielded a 52.5 Mbp genome with 8153 functionally annotated genes. Comparison with other sequenced green algae revealed unique protein families involved in osmotic stress tolerance and saccharide metabolism that support phenomic studies. Our results reveal the robust and flexible biology utilized by a green alga to successfully inhabit a desert coastline.

Original languageEnglish (US)
Article numbere25783
JournaleLife
Volume6
DOIs
StatePublished - Jun 17 2017

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Chlorophyta
Acclimatization
Algae
Genes
Genome
Carbon
United Arab Emirates
Desiccation
Metabolomics
Palmitates
Osmotic Pressure
Salinity
Metabolism
Sugars
Assays
Growth
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The genome and phenome of the green alga chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization. / Nelson, David; Khraiwesh, Basel; Fu, Weiqi; Alseekh, Saleh; Jaiswal, Ashish; Chaiboonchoe, Amphun; Hazzouri, Khaled M.; O'Connor, Matthew; Butterfoss, Glenn; Drou, Nizar; Rowe, Jillian D.; Harb, Jamil; Fernie, Alisdair R.; Gunsalus, Kristin; Salehi-Ashtiani, Kourosh.

In: eLife, Vol. 6, e25783, 17.06.2017.

Research output: Contribution to journalArticle

Nelson, David ; Khraiwesh, Basel ; Fu, Weiqi ; Alseekh, Saleh ; Jaiswal, Ashish ; Chaiboonchoe, Amphun ; Hazzouri, Khaled M. ; O'Connor, Matthew ; Butterfoss, Glenn ; Drou, Nizar ; Rowe, Jillian D. ; Harb, Jamil ; Fernie, Alisdair R. ; Gunsalus, Kristin ; Salehi-Ashtiani, Kourosh. / The genome and phenome of the green alga chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization. In: eLife. 2017 ; Vol. 6.
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AU - Rowe, Jillian D.

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