The forespore line of gene expression in Bacillus subtilis

Stephanie T. Wang, Barbara Setlow, Erin M. Conlon, Jessica L. Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger

Research output: Contribution to journalArticle

Abstract

Endospore formation by Bacillus subtilis involves three differentiating cell types, the predivisional cell, the mother cell, and the forespore. Here we report the program of gene expression in the forespore, which is governed by the RNA polymerase sigma factors σF and σG and the DNA-binding proteins RsfA and SpoVT. The σF factor turns on about 48 genes, including the gene for RsfA, which represses a gene in the σF regulon, and the gene for σG. The σG factor newly activates 81 genes, including the gene for SpoVT, which turns on (in nine cases) or stimulates (in 11 cases) the expression of 20 genes that had been turned on by σG and represses the expression of 27 others. The forespore line of gene expression consists of many genes that contribute to morphogenesis and to the resistance and germination properties of the spore but few that have metabolic functions. Comparative genomics reveals a core of genes in the σF and σG regulons that are widely conserved among endospore-forming species but are absent from closely related, but non-spore-forming Listeria spp. Two such partially conserved genes (ykoU and ykoV), which are members of the σG regulon, are shown to confer dry-heat resistance to dormant spores. The ykoV gene product, a homolog of the non-homologous end-joining protein Ku, is shown to associate with the nucleoid during germination. Extending earlier work on gene expression in the predivisional cell and the mother cell, we present an integrated overview of the entire program of sporulation gene expression.

Original languageEnglish (US)
Pages (from-to)16-37
Number of pages22
JournalJournal of Molecular Biology
Volume358
Issue number1
DOIs
StatePublished - Apr 21 2006

Fingerprint

Bacillus subtilis
Gene Expression
Genes
Regulon
Germination
Spores
Stem Cells
Sigma Factor
Listeria
DNA-Binding Proteins
Genomics
Morphogenesis
Hot Temperature

Keywords

  • Forespore
  • Promoters
  • Sigma factor
  • Sporulation
  • Transcriptional profiling

ASJC Scopus subject areas

  • Virology

Cite this

Wang, S. T., Setlow, B., Conlon, E. M., Lyon, J. L., Imamura, D., Sato, T., ... Eichenberger, P. (2006). The forespore line of gene expression in Bacillus subtilis. Journal of Molecular Biology, 358(1), 16-37. https://doi.org/10.1016/j.jmb.2006.01.059

The forespore line of gene expression in Bacillus subtilis. / Wang, Stephanie T.; Setlow, Barbara; Conlon, Erin M.; Lyon, Jessica L.; Imamura, Daisuke; Sato, Tsutomu; Setlow, Peter; Losick, Richard; Eichenberger, Patrick.

In: Journal of Molecular Biology, Vol. 358, No. 1, 21.04.2006, p. 16-37.

Research output: Contribution to journalArticle

Wang, ST, Setlow, B, Conlon, EM, Lyon, JL, Imamura, D, Sato, T, Setlow, P, Losick, R & Eichenberger, P 2006, 'The forespore line of gene expression in Bacillus subtilis', Journal of Molecular Biology, vol. 358, no. 1, pp. 16-37. https://doi.org/10.1016/j.jmb.2006.01.059
Wang ST, Setlow B, Conlon EM, Lyon JL, Imamura D, Sato T et al. The forespore line of gene expression in Bacillus subtilis. Journal of Molecular Biology. 2006 Apr 21;358(1):16-37. https://doi.org/10.1016/j.jmb.2006.01.059
Wang, Stephanie T. ; Setlow, Barbara ; Conlon, Erin M. ; Lyon, Jessica L. ; Imamura, Daisuke ; Sato, Tsutomu ; Setlow, Peter ; Losick, Richard ; Eichenberger, Patrick. / The forespore line of gene expression in Bacillus subtilis. In: Journal of Molecular Biology. 2006 ; Vol. 358, No. 1. pp. 16-37.
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