The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS

Elizabeth Cohen-Jonathan, Christine Toulas, Isabelle Ader, Sylvia Monteil, Cuider Allal, Jacques Bonnet, Andrew Hamilton, Said M. Sebti, Nicolas Daly-Schveitzer, Gilles Favre

Research output: Contribution to journalArticle

Abstract

In this paper, we describe the effect of the inhibitor of farnesyltransferase (FTI-277) on radioresistance induced by the 24-kDa isoform of FGF2 in human cells expressing wild-type RAS. Treatment with FTI- 277 (20 μM) for 48 h prior to irradiation led to a significant decrease in survival of radioresistant cells expressing the 24-kDa isoform (HeLa 3A) but had no effect on the survival of control cells (HeLa PINA). The radio- sensitizing effect of FTI-277 is accompanied by a stimulation of postmitotic cell death in HeLa 3A cells and by a reduction in G2/M-phase arrest in both cell types. These results dearly demonstrate that at least one farnesylated protein is involved in the regulation of the radioresistance induced by the 24-kDa isoform of FGF2. Furthermore, the radiation-induced G2/M-phase arrest is also under the control of farnesylated protein. This work also demonstrates that FTase inhibitors may be effective radio-sensitizers of certain human tumors with wild-type RAS.

Original languageEnglish (US)
Pages (from-to)404-411
Number of pages8
JournalRadiation Research
Volume152
Issue number4
DOIs
StatePublished - Oct 1999

Fingerprint

Farnesyltranstransferase
radiation resistance
Fibroblast Growth Factor 2
HeLa Cells
inhibitors
Protein Isoforms
G2 Phase
Radio
Cell Division
Cell Survival
cells
radio
mitosis
proteins
sensitizing
stimulation
death
Proteins
Cell Death
tumors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Cohen-Jonathan, E., Toulas, C., Ader, I., Monteil, S., Allal, C., Bonnet, J., ... Favre, G. (1999). The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS. Radiation Research, 152(4), 404-411. https://doi.org/10.2307/3580225

The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS. / Cohen-Jonathan, Elizabeth; Toulas, Christine; Ader, Isabelle; Monteil, Sylvia; Allal, Cuider; Bonnet, Jacques; Hamilton, Andrew; Sebti, Said M.; Daly-Schveitzer, Nicolas; Favre, Gilles.

In: Radiation Research, Vol. 152, No. 4, 10.1999, p. 404-411.

Research output: Contribution to journalArticle

Cohen-Jonathan, E, Toulas, C, Ader, I, Monteil, S, Allal, C, Bonnet, J, Hamilton, A, Sebti, SM, Daly-Schveitzer, N & Favre, G 1999, 'The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS', Radiation Research, vol. 152, no. 4, pp. 404-411. https://doi.org/10.2307/3580225
Cohen-Jonathan, Elizabeth ; Toulas, Christine ; Ader, Isabelle ; Monteil, Sylvia ; Allal, Cuider ; Bonnet, Jacques ; Hamilton, Andrew ; Sebti, Said M. ; Daly-Schveitzer, Nicolas ; Favre, Gilles. / The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS. In: Radiation Research. 1999 ; Vol. 152, No. 4. pp. 404-411.
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