The F-actin severing protein cofilin-1 is required for RNA polymerase II transcription elongation

Ales Obrdlik, Piergiorgio Percipalle

    Research output: Contribution to journalArticle

    Abstract

    In mammals actin contributes to transcription elongation by facilitating establishment of permissive chromatin. Here we report that the F-actin severing protein cofilin-1 is part of the same complex with actin and phosphorylated RNA polymerase (pol) II. In chromatin immunoprecipitation assays cofilin-1 was found selectively associated with transcribed regions of active genes, its occupancy being influenced by the polymerization state of actin. Cofilin-1 gene silencing led to a drop in FUrd incorporation into nascent transcripts. In cofilin-1 silenced cells chromatin immunoprecipitations showed that active genes were devoid of actin, phosphorylated pol II and displayed low histone H3 acetylation levels on K9. These findings suggest that cofilin-1 plays a major role in pol II transcription, facilitating association of elongating pol II and actin with active genes. We speculate that cofilin-1 performs its function in pol II transcription by regulating polymerization of gene-associated actin.

    Original languageEnglish (US)
    Pages (from-to)72-79
    Number of pages8
    JournalNucleus
    Volume2
    Issue number1
    DOIs
    StatePublished - Jan 1 2011

    Fingerprint

    Cofilin 1
    RNA Polymerase II
    Actins
    Proteins
    Chromatin Immunoprecipitation
    Polymerization
    Genes
    Gene Silencing
    Acetylation
    Histones
    Chromatin
    Mammals

    Keywords

    • Cofilin
    • Nascent mRNA
    • Nuclear actin
    • RNA polymerase II
    • Transcription elongation

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    The F-actin severing protein cofilin-1 is required for RNA polymerase II transcription elongation. / Obrdlik, Ales; Percipalle, Piergiorgio.

    In: Nucleus, Vol. 2, No. 1, 01.01.2011, p. 72-79.

    Research output: Contribution to journalArticle

    Obrdlik, Ales ; Percipalle, Piergiorgio. / The F-actin severing protein cofilin-1 is required for RNA polymerase II transcription elongation. In: Nucleus. 2011 ; Vol. 2, No. 1. pp. 72-79.
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