The extracellular matrix components laminin, fibronectin, and collagen IV are present among the epithelial cells forming Rathke’s pouch

Mark J. Horacek, J. C. Thompson, M. O. Dada, Louis Terracio

Research output: Contribution to journalArticle

Abstract

Cell-matrix interactions probably play a cooperative role with cell-hormone interactions to ensure normal differentiation of the adenohypophysis. The extracellular matrix (ECM) surrounding adult adenohypophysial cells contains laminin but its embryonic development has not been described. This study was carried out to test the hypothesis that adenohypophysial cells are associated with components in the ECM prior to cellular differentiation in the adenohypophysis. Fetuses were removed from Golden Syrian hamsters every 12 h from embryonic days 8.5-14. Coronal and sagittal 5-μm-thick sections of paraplast-embed- ded embryos were stained for fibronectin, laminin, or collagen IV using avidinbiotin immunoperoxidase staining. The adenohypophysial anlage was observed initially as a group of epithelial cells (Rathke’s pouch) in the roof of the stomatodeum in contact with a basement membrane. The basement membrane stained positively for laminin. collagen IV and fibronectin. Lighter staining for laminin. fibronectin and collagen IV was observed between the developing adenohypophysial cells in Rathke’s pouch. Proliferative activity was apparent in the anteroinferior region of Rathke’s pouch and resulted in the formation of the bulk of the adenohypophysis. Mesenchyme infiltrates the region between the base of Rathke’s pouch and the oral epithelium, thus separating the two. The basement membrane surrounding the pouch appears to become discontinuous in the regions of high proliferative activity. These results show that ECM components appear early during the development of adenohypophysial cells prior to their cellular differentiation into hormone-containing cells. This association between ECM components and developing adenohypophysial cells provides the anatomical basis for cell-ECM interactions to influence adenohypophysial development and differentiation.

Original languageEnglish (US)
Pages (from-to)69-74
Number of pages6
JournalCells Tissues Organs
Volume147
Issue number2
DOIs
StatePublished - 1993

Fingerprint

Laminin
Fibronectins
Extracellular Matrix
Collagen
Epithelial Cells
Anterior Pituitary Gland
Basement Membrane
Cell Communication
Hormones
Staining and Labeling
Mesocricetus
Mesoderm
Embryonic Development
Fetus
Embryonic Structures
Epithelium

Keywords

  • Collagen IV
  • Extracellular matrix
  • Fibronectin
  • Laminin
  • Rathke’s pouch

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

The extracellular matrix components laminin, fibronectin, and collagen IV are present among the epithelial cells forming Rathke’s pouch. / Horacek, Mark J.; Thompson, J. C.; Dada, M. O.; Terracio, Louis.

In: Cells Tissues Organs, Vol. 147, No. 2, 1993, p. 69-74.

Research output: Contribution to journalArticle

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