The eve stripe 2 enhancer employs multiple modes of transcriptional synergy

David N. Arnosti, Scott Barolo, Michael Levine, Stephen Small

Research output: Contribution to journalArticle

Abstract

Previous studies have provided a detailed model for the regulation of even-skipped (eve) stripe 2 expression in the Drosophila embryo. The bicoid (bcd) regulatory gradient triggers the expression of hunchback (hb); these work synergistically to activate the stripe in the anterior half of the embryo. bcd also coordinates the expression of two repressors, giant (gt) and Kruppel (Kr), which define the anterior and posterior borders of the stripe, respectively. Here, we report the findings of extensive cis- and trans- complementation analyses using a series of defective stripe 2 enhancers in transgenic embryos. This study reaches two primary conclusions. First, the stripe 2 enhancer is inherently 'sensitized' for repression by gt. We propose that gt specifies the sharp anterior stripe border by blocking two tiers of transcriptional synergy, cooperative binding to DNA and cooperative contact of bound activators with the transcription complex. Second, we find that the synergistic activity of hb and bcd is 'promiscuous'. For example, a maternally expressed Gal4-Sp1 fusion protein can functionally replace hb in the stripe 2 enhancer. This finding challenges previous proposals for dedicated hb and bcd interactions in the segmentation process.

Original languageEnglish (US)
Pages (from-to)205-214
Number of pages10
JournalDevelopment
Volume122
Issue number1
StatePublished - Jan 1996

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Embryonic Structures
Drosophila
DNA
Proteins

Keywords

  • Bicoid
  • Drosophila embryo
  • Even-skipped
  • Giant
  • Synergy
  • Transcription

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Arnosti, D. N., Barolo, S., Levine, M., & Small, S. (1996). The eve stripe 2 enhancer employs multiple modes of transcriptional synergy. Development, 122(1), 205-214.

The eve stripe 2 enhancer employs multiple modes of transcriptional synergy. / Arnosti, David N.; Barolo, Scott; Levine, Michael; Small, Stephen.

In: Development, Vol. 122, No. 1, 01.1996, p. 205-214.

Research output: Contribution to journalArticle

Arnosti, DN, Barolo, S, Levine, M & Small, S 1996, 'The eve stripe 2 enhancer employs multiple modes of transcriptional synergy', Development, vol. 122, no. 1, pp. 205-214.
Arnosti, David N. ; Barolo, Scott ; Levine, Michael ; Small, Stephen. / The eve stripe 2 enhancer employs multiple modes of transcriptional synergy. In: Development. 1996 ; Vol. 122, No. 1. pp. 205-214.
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