The effects of estrogen deficiency on cortical bone microporosity and mineralization

Divya Sharma, Adriana I. Larriera, Paolo E. Palacio-Mancheno, Vittorio Gatti, J. Christopher Fritton, Timothy Bromage, Luis Cardoso, Stephen B. Doty, Susannah P. Fritton

Research output: Contribution to journalArticle

Abstract

Recent studies have demonstrated matrix-mineral alterations in bone tissue surrounding osteocytes in estrogen-deficient animals. While cortical bone porosity has been shown to be a contributor to the mechanical properties of bone tissue, little analysis has been done to investigate the effects of estrogen deficiency on bone's microporosities, including the vascular and osteocyte lacunar porosities. In this study we examined alterations in cortical bone microporosity, mineralization, and cancellous bone architecture due to estrogen deficiency in the ovariectomized rat model of postmenopausal osteoporosis. Twenty-week-old female Sprague–Dawley rats were subjected to either ovariectomy or sham surgery. Six weeks post-surgery tibiae were analyzed using high-resolution micro-CT, backscattered electron imaging, nanoindentation, and dynamic histomorphometry. Estrogen deficiency caused an increase in cortical bone vascular porosity, with enlarged vascular pores and little change in tissue mineral density in the proximal tibial metaphysis. Measurements of cancellous architecture corresponded to previous studies reporting a decrease in bone volume fraction, an increase in trabecular separation, and a decrease in trabecular number in the proximal tibia due to estrogen deficiency. Nanoindentation results showed no differences in matrix stiffness in osteocyte-rich areas of the proximal tibia of estrogen-deficient rats, and bone labeling and backscattered electron imaging showed no significant changes in mineralization around the vascular pores. The findings demonstrate local surface alterations of vascular pores due to estrogen deficiency. An increase in cortical vascular porosity may diminish bone strength as well as alter bone mechanotransduction via interstitial fluid flow, both of which could contribute to bone fragility during postmenopausal osteoporosis.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalBone
Volume110
DOIs
StatePublished - May 1 2018

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Physiologic Calcification
Estrogens
Bone and Bones
Blood Vessels
Porosity
Osteocytes
Tibia
Postmenopausal Osteoporosis
Minerals
Electrons
Cortical Bone
Extracellular Fluid
Ovariectomy

Keywords

  • Bone mechanotransduction
  • Cortical porosity
  • Osteocyte lacunar porosity
  • Osteoporosis
  • Vascular porosity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Sharma, D., Larriera, A. I., Palacio-Mancheno, P. E., Gatti, V., Fritton, J. C., Bromage, T., ... Fritton, S. P. (2018). The effects of estrogen deficiency on cortical bone microporosity and mineralization. Bone, 110, 1-10. https://doi.org/10.1016/j.bone.2018.01.019

The effects of estrogen deficiency on cortical bone microporosity and mineralization. / Sharma, Divya; Larriera, Adriana I.; Palacio-Mancheno, Paolo E.; Gatti, Vittorio; Fritton, J. Christopher; Bromage, Timothy; Cardoso, Luis; Doty, Stephen B.; Fritton, Susannah P.

In: Bone, Vol. 110, 01.05.2018, p. 1-10.

Research output: Contribution to journalArticle

Sharma, D, Larriera, AI, Palacio-Mancheno, PE, Gatti, V, Fritton, JC, Bromage, T, Cardoso, L, Doty, SB & Fritton, SP 2018, 'The effects of estrogen deficiency on cortical bone microporosity and mineralization', Bone, vol. 110, pp. 1-10. https://doi.org/10.1016/j.bone.2018.01.019
Sharma D, Larriera AI, Palacio-Mancheno PE, Gatti V, Fritton JC, Bromage T et al. The effects of estrogen deficiency on cortical bone microporosity and mineralization. Bone. 2018 May 1;110:1-10. https://doi.org/10.1016/j.bone.2018.01.019
Sharma, Divya ; Larriera, Adriana I. ; Palacio-Mancheno, Paolo E. ; Gatti, Vittorio ; Fritton, J. Christopher ; Bromage, Timothy ; Cardoso, Luis ; Doty, Stephen B. ; Fritton, Susannah P. / The effects of estrogen deficiency on cortical bone microporosity and mineralization. In: Bone. 2018 ; Vol. 110. pp. 1-10.
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