The effect of oestrogen and alendronate therapies on postmenopausal bone loss around osseointegrated titanium implants

Gabriela Giro, Paulo Coelho, Rosa Maria Rodrigues Pereira, Vanda Jorgetti, Elcio Marcantonio, Silvana Regina Perez Orrico

Research output: Contribution to journalArticle

Abstract

Objectives: This study evaluated the influence of oestrogen deficiency and its therapies on bone tissue around osseointegrated implants. Methods: Implants were placed in 66 female rats tibiae. The animals were assigned into five groups: control (CTL), sham, ovariectomy (OVX), oestrogen (EST), and alendronate (ALE). While CTL was sacrificed 60 days after implant placement, other groups were subjected to ovariectomy or sham surgery according to group and euthanized after 90 days. Blood and urine samples were collected at sacrifice day for osteocalcin (OCN) and deoxypyridinoline (DPD) quantification. Densitometry of femur and lumbar vertebrae was performed in order to evaluate rats' skeletal impairment. Non-decalcified sections were referred to fluorescent and light microscopy for analyses of mineral apposition rate (MAR), eroded and osteoclastic surfaces, bone-to-implant contact (BIC), and bone area fraction occupancy (BAFO). Results: Results from the OVX group showed significantly lower bone mineral density (BMD), BIC, BAFO, and MAR, while OCN, deoxipiridinoline, eroded surface and ostecoclastic surface were increased compared with the other groups of the study. ALE reduced OCN and DPD concentrations, MAR, osteoclastic and eroded surfaces, and no difference was in BIC and BAFO relative to SHAM. EST and CTL showed similar results to SHAM for measurements. Conclusions: Oestrogen deficiency exerted a negative influence on bone tissue around implants, while oestrogen replacement therapy and alendronate were effective against its effects. Although alendronate therapy maintained the quantity of bone around implants, studies evaluating bone turnover kinetics are warranted.

Original languageEnglish (US)
Pages (from-to)259-264
Number of pages6
JournalClinical Oral Implants Research
Volume22
Issue number3
DOIs
StatePublished - Mar 2011

Fingerprint

Alendronate
Postmenopausal Osteoporosis
Titanium
Estrogens
Bone and Bones
Osteocalcin
Minerals
Therapeutics
Ovariectomy
Lumbar Vertebrae
Estrogen Replacement Therapy
Densitometry
Bone Remodeling
Tibia
Bone Density
Femur
Microscopy
Urine
Light
Control Groups

Keywords

  • Alendronate
  • Dental implants
  • Oestrogen replacement therapy
  • Osteoporosis
  • Ovariectomy
  • Rats

ASJC Scopus subject areas

  • Oral Surgery

Cite this

The effect of oestrogen and alendronate therapies on postmenopausal bone loss around osseointegrated titanium implants. / Giro, Gabriela; Coelho, Paulo; Pereira, Rosa Maria Rodrigues; Jorgetti, Vanda; Marcantonio, Elcio; Orrico, Silvana Regina Perez.

In: Clinical Oral Implants Research, Vol. 22, No. 3, 03.2011, p. 259-264.

Research output: Contribution to journalArticle

Giro, Gabriela ; Coelho, Paulo ; Pereira, Rosa Maria Rodrigues ; Jorgetti, Vanda ; Marcantonio, Elcio ; Orrico, Silvana Regina Perez. / The effect of oestrogen and alendronate therapies on postmenopausal bone loss around osseointegrated titanium implants. In: Clinical Oral Implants Research. 2011 ; Vol. 22, No. 3. pp. 259-264.
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