The effect of laminin-1-doped nanoroughened implant surfaces: Gene expression and morphological evaluation

Humberto Osvaldo Schwartz-Filho, Kostas Bougas, Paulo G. Coelho, Ying Xue, Mariko Hayashi, Rafael Silveira Faeda, Rosemary Adriana Chiérici Marcantonio, Daisuke Ono, Fumio Kobayashi, Kamal Mustafa, Ann Wennerberg, Ryo Jimbo

Research output: Contribution to journalArticle

Abstract

Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 g/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface.

Original languageEnglish (US)
Article number305638
JournalInternational Journal of Biomaterials
DOIs
StatePublished - Dec 1 2012

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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    Schwartz-Filho, H. O., Bougas, K., Coelho, P. G., Xue, Y., Hayashi, M., Faeda, R. S., Marcantonio, R. A. C., Ono, D., Kobayashi, F., Mustafa, K., Wennerberg, A., & Jimbo, R. (2012). The effect of laminin-1-doped nanoroughened implant surfaces: Gene expression and morphological evaluation. International Journal of Biomaterials, [305638]. https://doi.org/10.1155/2012/305638