The effect of a cAMP analogue on Ca2+ ionophore-, antigen- and agonist-induced inositol phosphate release in rat basophilic leukemia (RBL-1) cells

S. H.J. Galadari, H. R. Morris, V. Di Marzo

Research output: Contribution to journalArticle

Abstract

The effect of the stable cAMP analogue 8-Br-cAMP on leukotriene D4 (LTD4)-, 5′-N-ethyl-carboxamidoadenosine (NECA)-, antigen- and Ca2+ ionophore-induced inositol phosphate (IP) production was studied in RBL-1 cells. The cAMP analogue significantly inhibited LTD4- and antigen induced-1P production, thus supporting the hypothesis of a negative interaction between cAMP and phosphoinositide breakdown in blood cells. Ionophore-induced IP release, which was blocked by a 5-lipoxygenase inhibitor and by a LT-receptor antagonist, and therefore is probably mediated by LTs, was also inhibited by 8-Br-cAMP. NECA-induced IP release was not significantly inhibited by the cyclic nucleotide, thus showing that the effect described herein is not a general action on receptor-activated phospholipase C. 8-Br-cAMP did, however, inhibit GTPγS-induced IP release in permeabilised RBL-1 cells, thus suggesting that the inhibition does not occur at the receptor level but might be due, at least in part, to an effect on some receptor-coupled G proteins.

Original languageEnglish (US)
Pages (from-to)218-222
Number of pages5
JournalBBA - Molecular Cell Research
Volume1133
Issue number2
DOIs
StatePublished - Jan 13 1992

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Keywords

  • (Rat RBL-1 cell)
  • Agonist
  • Antigen
  • Calcium ionophore
  • Inositol phosphate
  • cyclic AMP

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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