Abstract
Light and electron microscopy revealed that there are both rods and cones in the retina of the eel Anguilla rostrata. The rods predominate with a rod to cone ratio of 150:1. The spectral sensitivity of the dark-adapted eyecup ERG had a peak at about 520 nm and was well fit by a vitamin A2 nomogram pigment with a λ(max) = 520 nm. This agrees with the eel photopigment measurements of other investigators. This result implies that a single spectral mechanism - the rods- provides the input for the dark-adapted ERG. The spectral sensitivity of the ERG to flicker in the light-adapted eyecup preparation was shifted to longer wavelengths; it peaked at around 550 nm. However, there was evidence that this technique might not have completely eliminated rod intrusion. Rod responses were abolished in a bleached isolated retina preparation, in which it was shown that there were two classes of cone-like mechanisms, one with λ(max) of 550 nm and the other with λ(max) of <450 nm. Ganglion cell recording provided preliminary evidence for opponent-color processing. Horizontal cells were only of the L type with both rod and cone inputs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 123-138 |
| Number of pages | 16 |
| Journal | Journal of General Physiology |
| Volume | 71 |
| Issue number | 2 |
| State | Published - 1978 |
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ASJC Scopus subject areas
- Physiology
Cite this
The eel retina. Receptor classes and spectral mechanisms. / Gordon, J.; Shapley, Robert; Kaplan, E.
In: Journal of General Physiology, Vol. 71, No. 2, 1978, p. 123-138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The eel retina. Receptor classes and spectral mechanisms
AU - Gordon, J.
AU - Shapley, Robert
AU - Kaplan, E.
PY - 1978
Y1 - 1978
N2 - Light and electron microscopy revealed that there are both rods and cones in the retina of the eel Anguilla rostrata. The rods predominate with a rod to cone ratio of 150:1. The spectral sensitivity of the dark-adapted eyecup ERG had a peak at about 520 nm and was well fit by a vitamin A2 nomogram pigment with a λ(max) = 520 nm. This agrees with the eel photopigment measurements of other investigators. This result implies that a single spectral mechanism - the rods- provides the input for the dark-adapted ERG. The spectral sensitivity of the ERG to flicker in the light-adapted eyecup preparation was shifted to longer wavelengths; it peaked at around 550 nm. However, there was evidence that this technique might not have completely eliminated rod intrusion. Rod responses were abolished in a bleached isolated retina preparation, in which it was shown that there were two classes of cone-like mechanisms, one with λ(max) of 550 nm and the other with λ(max) of <450 nm. Ganglion cell recording provided preliminary evidence for opponent-color processing. Horizontal cells were only of the L type with both rod and cone inputs.
AB - Light and electron microscopy revealed that there are both rods and cones in the retina of the eel Anguilla rostrata. The rods predominate with a rod to cone ratio of 150:1. The spectral sensitivity of the dark-adapted eyecup ERG had a peak at about 520 nm and was well fit by a vitamin A2 nomogram pigment with a λ(max) = 520 nm. This agrees with the eel photopigment measurements of other investigators. This result implies that a single spectral mechanism - the rods- provides the input for the dark-adapted ERG. The spectral sensitivity of the ERG to flicker in the light-adapted eyecup preparation was shifted to longer wavelengths; it peaked at around 550 nm. However, there was evidence that this technique might not have completely eliminated rod intrusion. Rod responses were abolished in a bleached isolated retina preparation, in which it was shown that there were two classes of cone-like mechanisms, one with λ(max) of 550 nm and the other with λ(max) of <450 nm. Ganglion cell recording provided preliminary evidence for opponent-color processing. Horizontal cells were only of the L type with both rod and cone inputs.
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M3 - Article
VL - 71
SP - 123
EP - 138
JO - Journal of General Physiology
JF - Journal of General Physiology
SN - 0022-1295
IS - 2
ER -