The dynamic activation of colicin Ia channels in planar bilayer lipid membrane

R. Cassia-Moura, A. Popescu, J. R S A Lima, C. A S Andrade, L. S. Ventura, K. S A Lima, J. Rinzel

Research output: Contribution to journalArticle

Abstract

Dynamic activation of ion channels formed by colicin Ia incorporated into a planar bilayer lipid membrane (BLM) was investigated by the voltage clamp technique using different step voltage stimuli. We have demonstrated a critical resting interval, Δt(c), between two identical successive voltage pulses. If the second pulse is applied within Δt(c), it produces a predictable current response. On the contrary, if the second pulse is applied after Δt(c), the current response cannot be reliably predicted. Computer simulations based on an idealized mathematical model, developed in this paper, qualitatively reproduce the system's dynamic responses to stimulus trains. The behavior of the ion channels, when the resting period exceeds Δt(c), may be interpreted as a transient gain or loss or resetting of memory, as revealed by a specific sequence of electrical pulses used for stimulation. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)235-241
Number of pages7
JournalJournal of Theoretical Biology
Volume206
Issue number2
DOIs
StatePublished - Sep 21 2000

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colicins
Colicins
Lipid Bilayer
lipid bilayers
Lipid bilayers
ion channels
Lipid Bilayers
Membrane Lipids
Ion Channels
Activation
Membrane
Chemical activation
patch-clamp technique
resting periods
Voltage
Electric potential
Patch-Clamp Techniques
computer simulation
Computer Simulation
Theoretical Models

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Cassia-Moura, R., Popescu, A., Lima, J. R. S. A., Andrade, C. A. S., Ventura, L. S., Lima, K. S. A., & Rinzel, J. (2000). The dynamic activation of colicin Ia channels in planar bilayer lipid membrane. Journal of Theoretical Biology, 206(2), 235-241. https://doi.org/10.1006/jtbi.2000.2124

The dynamic activation of colicin Ia channels in planar bilayer lipid membrane. / Cassia-Moura, R.; Popescu, A.; Lima, J. R S A; Andrade, C. A S; Ventura, L. S.; Lima, K. S A; Rinzel, J.

In: Journal of Theoretical Biology, Vol. 206, No. 2, 21.09.2000, p. 235-241.

Research output: Contribution to journalArticle

Cassia-Moura, R, Popescu, A, Lima, JRSA, Andrade, CAS, Ventura, LS, Lima, KSA & Rinzel, J 2000, 'The dynamic activation of colicin Ia channels in planar bilayer lipid membrane', Journal of Theoretical Biology, vol. 206, no. 2, pp. 235-241. https://doi.org/10.1006/jtbi.2000.2124
Cassia-Moura R, Popescu A, Lima JRSA, Andrade CAS, Ventura LS, Lima KSA et al. The dynamic activation of colicin Ia channels in planar bilayer lipid membrane. Journal of Theoretical Biology. 2000 Sep 21;206(2):235-241. https://doi.org/10.1006/jtbi.2000.2124
Cassia-Moura, R. ; Popescu, A. ; Lima, J. R S A ; Andrade, C. A S ; Ventura, L. S. ; Lima, K. S A ; Rinzel, J. / The dynamic activation of colicin Ia channels in planar bilayer lipid membrane. In: Journal of Theoretical Biology. 2000 ; Vol. 206, No. 2. pp. 235-241.
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