The Drosophila dorsal morphogen represses the tolloid gene by interacting with a silencer element

Nikolai Kirov, Steve Childs, Michael O'Connor, Christine Rushlow

Research output: Contribution to journalArticle

Abstract

The dorsal protein (DL) regulates the transcriptional activity of several genes that determine cell fate along the dorsoventral axis of the Drosophila melanogaster embryo. DL is present at high levels in ventral nuclei, where it activates some genes (twi and sna) and represses others (zen, dpp, and tld). DL shows homology to the Rel family of proteins and interacts with specific DNA sequences in the regulatory regions of its target genes. The distal portion of the zen gene acts as a silencer that can mediate the repression of a heterologous promoter in ventral regions of the embryo. It contains four DL binding sites which alone are sufficient for activation but not repression. Here we analyze the interaction of DL with another one of its repressed targets, the tolloid (tld) gene. Approximately 800 bp of 5′-flanking sequences upstream of the tld coding region were shown to drive an expression pattern indistinguishable from the wild-type pattern. A 423-bp fragment located within these sequences contains two DL binding sites and was shown to act as a silencer to mediate ventral repression. Point mutations in the sites abolish not only DNA binding but also ventral repression. We discuss a comparison of the DNA sequences from the zen and tld promoters and the possible mechanisms of transcriptional silencing.

Original languageEnglish (US)
Pages (from-to)713-722
Number of pages10
JournalMolecular and Cellular Biology
Volume14
Issue number1
StatePublished - 1994

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Transcriptional Silencer Elements
Drosophila
Genes
Proteins
Protein Binding
Embryonic Structures
Binding Sites
5' Flanking Region
Nucleic Acid Regulatory Sequences
Drosophila melanogaster
Point Mutation
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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The Drosophila dorsal morphogen represses the tolloid gene by interacting with a silencer element. / Kirov, Nikolai; Childs, Steve; O'Connor, Michael; Rushlow, Christine.

In: Molecular and Cellular Biology, Vol. 14, No. 1, 1994, p. 713-722.

Research output: Contribution to journalArticle

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