The dorsal morphogen is a sequence-specific DNA-binding protein that interacts with a long-range repression element in drosophila

Y. Tony Ip, Rachel Kraut, Michael Levine, Christine Rushlow

Research output: Contribution to journalArticle

Abstract

A gradient of the maternal morphogen dorsal (dl) establishes dorsal-ventral (D-V) polarity in the early Drosophila embryo. The dl concentration gradient is initiated by regulated nuclear transport, and only protein that enters nuclei is active in the D-V patterning process. Here we show that dl is a DNA-binding protein that specifically interacts with distal sequences of the zerknüllt (zen) promoter, one of the genetic targets of the morphogen. These zen sequences have the properties of a silencer element and can act over long distances to repress the expression of a heterologous promoter. The dl protein recognizes a sequence motif similar to that of the mammalian transcriptional activator NF-κB, which was shown to contain extensive homology with dl and the oncoprotein rel. We present evidence that the DNA-binding activity of the dl protein is mediated by the region of homology (the rel domain) conserved in the rel and NF-κB proteins.

Original languageEnglish (US)
Pages (from-to)439-446
Number of pages8
JournalCell
Volume64
Issue number2
DOIs
StatePublished - Jan 25 1991

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DNA-Binding Proteins
Drosophila
Transcriptional Silencer Elements
Cell Nucleus Active Transport
Oncogene Proteins
Genetic Promoter Regions
Carrier Proteins
Proteins
Embryonic Structures
Mothers
DNA
IgA receptor

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The dorsal morphogen is a sequence-specific DNA-binding protein that interacts with a long-range repression element in drosophila. / Tony Ip, Y.; Kraut, Rachel; Levine, Michael; Rushlow, Christine.

In: Cell, Vol. 64, No. 2, 25.01.1991, p. 439-446.

Research output: Contribution to journalArticle

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