The distinct temporal origins of olfactory bulb interneuron subtypes

Renata Batista-Brito, Jennie Close, Robert Machold, Gordon Fishell

    Research output: Contribution to journalArticle

    Abstract

    Olfactory bulb (OB) interneurons are a heterogeneous population produced beginning in embryogenesis and continuing through adulthood. Understanding how this diversity arises will provide insight into how OB microcircuitry is established as well as adult neurogenesis. Particular spatial domains have been shown to contribute specific interneuron subtypes. However, the temporal profile by which OB interneuron subtypes are produced is unknown. Using inducible genetic fate mapping of Dlx1/2 precursors, we analyzed the production of seven OB interneuron subtypes and found that the generation of each subpopulation has a unique temporal signature. Within the glomerular layer, the production of tyrosine hydroxylase-positive interneurons is maximal during early embryogenesis and decreases thereafter. In contrast, the generation of calbindin interneurons is maximal during late embryogenesis and declines postnatally, whereas calretinin (CR) cell production is low during embryogenesis and increases postnatally. Parvalbumin interneurons within the external plexiform layer are produced only perinatally, whereas the generation of 5T4-positive granule cells in the mitral cell layer does not change significantly over time. CR-positive granule cells are not produced at early embryonic time points, but constitute a large percentage of the granule cells born after birth. Blanes cells in contrast are produced in greatest number during embryogenesis. Together we provide the first comprehensive analysis of the temporal generation of OB interneuron subtypes and demonstrate that the timing by which these populations are produced is tightly orchestrated.

    Original languageEnglish (US)
    Pages (from-to)3966-3975
    Number of pages10
    JournalJournal of Neuroscience
    Volume28
    Issue number15
    DOIs
    StatePublished - Apr 9 2008

    Fingerprint

    Olfactory Bulb
    Interneurons
    Embryonic Development
    Calbindin 2
    Calbindins
    Parvalbumins
    Neurogenesis
    Tyrosine 3-Monooxygenase
    Population
    Parturition

    Keywords

    • Adult stem cells
    • Genetic fate mapping
    • Interneurons
    • Neurogenesis
    • Neuronal birthdate
    • Olfactory bulb

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    The distinct temporal origins of olfactory bulb interneuron subtypes. / Batista-Brito, Renata; Close, Jennie; Machold, Robert; Fishell, Gordon.

    In: Journal of Neuroscience, Vol. 28, No. 15, 09.04.2008, p. 3966-3975.

    Research output: Contribution to journalArticle

    Batista-Brito, R, Close, J, Machold, R & Fishell, G 2008, 'The distinct temporal origins of olfactory bulb interneuron subtypes', Journal of Neuroscience, vol. 28, no. 15, pp. 3966-3975. https://doi.org/10.1523/JNEUROSCI.5625-07.2008
    Batista-Brito, Renata ; Close, Jennie ; Machold, Robert ; Fishell, Gordon. / The distinct temporal origins of olfactory bulb interneuron subtypes. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 15. pp. 3966-3975.
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