The cytoskeleton of human polymorphonuclear leukocytes

Phagocytosis and degranulation

M. I. Ryder, Richard Niederman, E. J. Taggart

Research output: Contribution to journalArticle

Abstract

Current evidence indicates that polymorphonuclear leukocyte (PMN) chemotaxis and phagocytosis are effected by an actin-myosin contractile system. However, the structural relationship of the contractile cytoskeleton to cell motility is still in question. In addition, while evidence suggests that microtubules are responsible for orientation during chemotaxis, the role of microtubules in degranulation is unresolved. To determine the organizational relationship between these cytoskeletal elements and phagocytosis, we examined whole-mount preparations of PMNs engulfing bacteria. These preparations were examined in the transmission electron microscope (EM) and photographed as stereo pairs. Two important observations were made. First, there was an increased density of cytoskeletal elements in the pseudopod surrounding bacteria. Second, microtubule elements were intimately associated with lysosomal granules, vesicles, and phagosomes. Lysosomal granules and vesicles aligned along microtubules and clustered around phagosomes. This suggests that the microtubules may provide a tracking mechanism whereby lysosomes are specifically parceled out to phagocytic vascuoles. These results also suggest that phagocytosis and degranulation may involve different effector mechanisms.

Original languageEnglish (US)
Pages (from-to)317-327
Number of pages11
JournalAnatomical Record
Volume203
Issue number3
StatePublished - 1982

Fingerprint

chemotaxis
vesicle
cytoskeleton
Cytoskeleton
phagocytosis
Phagocytosis
Microtubules
microtubules
neutrophils
Neutrophils
bacterium
motility
phagosomes
Phagosomes
granules
electron
Leukocyte Chemotaxis
pseudopodia
Bacteria
Pseudopodia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

The cytoskeleton of human polymorphonuclear leukocytes : Phagocytosis and degranulation. / Ryder, M. I.; Niederman, Richard; Taggart, E. J.

In: Anatomical Record, Vol. 203, No. 3, 1982, p. 317-327.

Research output: Contribution to journalArticle

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