The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration

Sarah V. Gerhart, Diane M. Eble, R. Michael Burger, Stefan N. Oline, Ana Vacaru, Kirsten Sadler Edepli, Rebecca Jefferis, M. Kathryn Iovine

Research output: Contribution to journalArticle

Abstract

Connexins (Cx) are the subunits of gap junctions, membraneous protein channels that permit the exchange of small molecules between adjacent cells. Cx43 is required for cell proliferation in the zebrafish caudal fin. Previously, we found that a Cx43-like connexin, cx40.8, is co-expressed with cx43 in the population of proliferating cells during fin regeneration. Here we demonstrate that Cx40.8 exhibits novel differential subcellular localization in vivo, depending on the growth status of the fin. During fin ontogeny, Cx40.8 is found at the plasma membrane, but Cx40.8 is retained in the Golgi apparatus during regeneration. We next identified a 30 amino acid domain of Cx40.8 responsible for its dynamic localization. One possible explanation for the differential localization is that Cx40.8 contributes to the regulation of Cx43 in vivo, perhaps modifying channel activity during ontogenetic growth. However, we find that the voltage-gating properties of Cx40.8 are similar to Cx43. Together our findings reveal that Cx40.8 exhibits differential subcellular localization in vivo, dependent on a discrete domain in its carboxy terminus. We suggest that the dynamic localization of Cx40.8 differentially influences Cx43-dependent cell proliferation during ontogeny and regeneration.

Original languageEnglish (US)
Article numbere31364
JournalPLoS One
Volume7
Issue number2
DOIs
StatePublished - Feb 8 2012

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connexins
Connexin 43
Connexins
fins
ontogeny
Regeneration
cell proliferation
Proteins
proteins
Cell proliferation
Golgi apparatus
Danio rerio
Cell Proliferation
plasma membrane
Golgi Apparatus
Zebrafish
cells
Cell membranes
Growth
amino acids

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration. / Gerhart, Sarah V.; Eble, Diane M.; Burger, R. Michael; Oline, Stefan N.; Vacaru, Ana; Sadler Edepli, Kirsten; Jefferis, Rebecca; Iovine, M. Kathryn.

In: PLoS One, Vol. 7, No. 2, e31364, 08.02.2012.

Research output: Contribution to journalArticle

Gerhart, Sarah V. ; Eble, Diane M. ; Burger, R. Michael ; Oline, Stefan N. ; Vacaru, Ana ; Sadler Edepli, Kirsten ; Jefferis, Rebecca ; Iovine, M. Kathryn. / The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration. In: PLoS One. 2012 ; Vol. 7, No. 2.
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