The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration

Sarah V. Gerhart, Diane M. Eble, R. Michael Burger, Stefan N. Oline, Ana Vacaru, Kirsten Sadler Edepli, Rebecca Jefferis, M. Kathryn Iovine

    Research output: Contribution to journalArticle

    Abstract

    Connexins (Cx) are the subunits of gap junctions, membraneous protein channels that permit the exchange of small molecules between adjacent cells. Cx43 is required for cell proliferation in the zebrafish caudal fin. Previously, we found that a Cx43-like connexin, cx40.8, is co-expressed with cx43 in the population of proliferating cells during fin regeneration. Here we demonstrate that Cx40.8 exhibits novel differential subcellular localization in vivo, depending on the growth status of the fin. During fin ontogeny, Cx40.8 is found at the plasma membrane, but Cx40.8 is retained in the Golgi apparatus during regeneration. We next identified a 30 amino acid domain of Cx40.8 responsible for its dynamic localization. One possible explanation for the differential localization is that Cx40.8 contributes to the regulation of Cx43 in vivo, perhaps modifying channel activity during ontogenetic growth. However, we find that the voltage-gating properties of Cx40.8 are similar to Cx43. Together our findings reveal that Cx40.8 exhibits differential subcellular localization in vivo, dependent on a discrete domain in its carboxy terminus. We suggest that the dynamic localization of Cx40.8 differentially influences Cx43-dependent cell proliferation during ontogeny and regeneration.

    Original languageEnglish (US)
    Article numbere31364
    JournalPLoS One
    Volume7
    Issue number2
    DOIs
    StatePublished - Feb 8 2012

    Fingerprint

    connexins
    Connexin 43
    Connexins
    fins
    ontogeny
    Regeneration
    cell proliferation
    Proteins
    proteins
    Cell proliferation
    Golgi apparatus
    Danio rerio
    Cell Proliferation
    plasma membrane
    Golgi Apparatus
    Zebrafish
    cells
    Cell membranes
    Growth
    amino acids

    ASJC Scopus subject areas

    • Medicine(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Cite this

    Gerhart, S. V., Eble, D. M., Burger, R. M., Oline, S. N., Vacaru, A., Sadler Edepli, K., ... Iovine, M. K. (2012). The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration. PLoS One, 7(2), [e31364]. https://doi.org/10.1371/journal.pone.0031364

    The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration. / Gerhart, Sarah V.; Eble, Diane M.; Burger, R. Michael; Oline, Stefan N.; Vacaru, Ana; Sadler Edepli, Kirsten; Jefferis, Rebecca; Iovine, M. Kathryn.

    In: PLoS One, Vol. 7, No. 2, e31364, 08.02.2012.

    Research output: Contribution to journalArticle

    Gerhart, SV, Eble, DM, Burger, RM, Oline, SN, Vacaru, A, Sadler Edepli, K, Jefferis, R & Iovine, MK 2012, 'The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration', PLoS One, vol. 7, no. 2, e31364. https://doi.org/10.1371/journal.pone.0031364
    Gerhart, Sarah V. ; Eble, Diane M. ; Burger, R. Michael ; Oline, Stefan N. ; Vacaru, Ana ; Sadler Edepli, Kirsten ; Jefferis, Rebecca ; Iovine, M. Kathryn. / The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration. In: PLoS One. 2012 ; Vol. 7, No. 2.
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