The completion of the Mammalian Gene Collection

Gary Temple, Daniela S. Gerhard, Rebekah Rasooly, Elise A. Feingold, Peter J. Good, Cristen Robinson, Allison Mandich, Jeffrey G. Derge, Jeanne Lewis, Debonny Shoaf, Francis S. Collins, Wonhee Jang, Lukas Wagner, Carolyn M. Shenmen, Leonie Misquitta, Carl F. Schaefer, Kenneth H. Buetow, Tom I. Bonner, Linda Yankie, Ming Ward & 107 others Lon Phan, Alex Astashyn, Garth Brown, Catherine Farrell, Jennifer Hart, Melissa Landrum, Bonnie L. Maidak, Michael Murphy, Terence Murphy, Bhanu Rajput, Lillian Riddick, David Webb, Janet Weber, Wendy Wu, Kim D. Pruitt, Donna Maglott, Adam Siepel, Brona Brejova, Mark Diekhans, Rachel Harte, Robert Baertsch, Jim Kent, David Haussler, Michael Brent, Laura Langton, Charles L.G. Comstock, Michael Stevens, Chaochun Wei, Marijke J. Van Baren, Kourosh Salehi-Ashtiani, Ryan R. Murray, Lila Ghamsari, Elizabeth Mello, Chenwei Lin, Christa Pennacchio, Kirsten Schreiber, Nicole Shapiro, Amber Marsh, Elizabeth Pardes, Troy Moore, Anita Lebeau, Mike Muratet, Blake Simmons, David Kloske, Stephanie Sieja, James Hudson, Praveen Sethupathy, Michael Brownstein, Narayan Bhat, Joseph Lazar, Howard Jacob, Chris E. Gruber, Mark R. Smith, John McPherson, Angela M. Garcia, Preethi H. Gunaratne, Jiaqian Wu, Donna Muzny, Richard A. Gibbs, Alice C. Young, Gerard G. Bouffard, Robert W. Blakesley, Jim Mullikin, Eric D. Green, Mark C. Dickson, Alex C. Rodriguez, Jane Grimwood, Jeremy Schmutz, Richard M. Myers, Martin Hirst, Thomas Zeng, Kane Tse, Michelle Moksa, Merinda Deng, Kevin Ma, Diana Mah, Johnson Pang, Greg Taylor, Eric Chuah, Athena Deng, Keith Fichter, Anne Go, Stephanie Lee, Jing Wang, Malachi Griffith, Ryan Morin, Richard A. Moore, Michael Mayo, Sarah Munro, Susan Wagner, Steven J.M. Jones, Robert A. Holt, Marco A. Marra, Sun Lu, Shuwei Yang, James Hartigan, Marcus Graf, Ralf Wagner, Stanley Letovksy, Jacqueline C. Pulido, Keith Robison, Dominic Esposito, James Hartley, Vanessa E. Wall, Ralph F. Hopkins, Osamu Ohara, Stefan Wiemann

    Research output: Contribution to journalArticle

    Abstract

    Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.

    Original languageEnglish (US)
    Pages (from-to)2324-2333
    Number of pages10
    JournalGenome Research
    Volume19
    Issue number12
    DOIs
    StatePublished - Dec 1 2009

    Fingerprint

    Genes
    Clone Cells
    Organism Cloning
    Expressed Sequence Tags
    Gene Library
    PubMed
    Publications
    Proteins
    Complementary DNA
    Research Personnel
    Polymerase Chain Reaction
    DNA

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

    Cite this

    Temple, G., Gerhard, D. S., Rasooly, R., Feingold, E. A., Good, P. J., Robinson, C., ... Wiemann, S. (2009). The completion of the Mammalian Gene Collection. Genome Research, 19(12), 2324-2333. https://doi.org/10.1101/gr.095976.109

    The completion of the Mammalian Gene Collection. / Temple, Gary; Gerhard, Daniela S.; Rasooly, Rebekah; Feingold, Elise A.; Good, Peter J.; Robinson, Cristen; Mandich, Allison; Derge, Jeffrey G.; Lewis, Jeanne; Shoaf, Debonny; Collins, Francis S.; Jang, Wonhee; Wagner, Lukas; Shenmen, Carolyn M.; Misquitta, Leonie; Schaefer, Carl F.; Buetow, Kenneth H.; Bonner, Tom I.; Yankie, Linda; Ward, Ming; Phan, Lon; Astashyn, Alex; Brown, Garth; Farrell, Catherine; Hart, Jennifer; Landrum, Melissa; Maidak, Bonnie L.; Murphy, Michael; Murphy, Terence; Rajput, Bhanu; Riddick, Lillian; Webb, David; Weber, Janet; Wu, Wendy; Pruitt, Kim D.; Maglott, Donna; Siepel, Adam; Brejova, Brona; Diekhans, Mark; Harte, Rachel; Baertsch, Robert; Kent, Jim; Haussler, David; Brent, Michael; Langton, Laura; Comstock, Charles L.G.; Stevens, Michael; Wei, Chaochun; Van Baren, Marijke J.; Salehi-Ashtiani, Kourosh; Murray, Ryan R.; Ghamsari, Lila; Mello, Elizabeth; Lin, Chenwei; Pennacchio, Christa; Schreiber, Kirsten; Shapiro, Nicole; Marsh, Amber; Pardes, Elizabeth; Moore, Troy; Lebeau, Anita; Muratet, Mike; Simmons, Blake; Kloske, David; Sieja, Stephanie; Hudson, James; Sethupathy, Praveen; Brownstein, Michael; Bhat, Narayan; Lazar, Joseph; Jacob, Howard; Gruber, Chris E.; Smith, Mark R.; McPherson, John; Garcia, Angela M.; Gunaratne, Preethi H.; Wu, Jiaqian; Muzny, Donna; Gibbs, Richard A.; Young, Alice C.; Bouffard, Gerard G.; Blakesley, Robert W.; Mullikin, Jim; Green, Eric D.; Dickson, Mark C.; Rodriguez, Alex C.; Grimwood, Jane; Schmutz, Jeremy; Myers, Richard M.; Hirst, Martin; Zeng, Thomas; Tse, Kane; Moksa, Michelle; Deng, Merinda; Ma, Kevin; Mah, Diana; Pang, Johnson; Taylor, Greg; Chuah, Eric; Deng, Athena; Fichter, Keith; Go, Anne; Lee, Stephanie; Wang, Jing; Griffith, Malachi; Morin, Ryan; Moore, Richard A.; Mayo, Michael; Munro, Sarah; Wagner, Susan; Jones, Steven J.M.; Holt, Robert A.; Marra, Marco A.; Lu, Sun; Yang, Shuwei; Hartigan, James; Graf, Marcus; Wagner, Ralf; Letovksy, Stanley; Pulido, Jacqueline C.; Robison, Keith; Esposito, Dominic; Hartley, James; Wall, Vanessa E.; Hopkins, Ralph F.; Ohara, Osamu; Wiemann, Stefan.

    In: Genome Research, Vol. 19, No. 12, 01.12.2009, p. 2324-2333.

    Research output: Contribution to journalArticle

    Temple, G, Gerhard, DS, Rasooly, R, Feingold, EA, Good, PJ, Robinson, C, Mandich, A, Derge, JG, Lewis, J, Shoaf, D, Collins, FS, Jang, W, Wagner, L, Shenmen, CM, Misquitta, L, Schaefer, CF, Buetow, KH, Bonner, TI, Yankie, L, Ward, M, Phan, L, Astashyn, A, Brown, G, Farrell, C, Hart, J, Landrum, M, Maidak, BL, Murphy, M, Murphy, T, Rajput, B, Riddick, L, Webb, D, Weber, J, Wu, W, Pruitt, KD, Maglott, D, Siepel, A, Brejova, B, Diekhans, M, Harte, R, Baertsch, R, Kent, J, Haussler, D, Brent, M, Langton, L, Comstock, CLG, Stevens, M, Wei, C, Van Baren, MJ, Salehi-Ashtiani, K, Murray, RR, Ghamsari, L, Mello, E, Lin, C, Pennacchio, C, Schreiber, K, Shapiro, N, Marsh, A, Pardes, E, Moore, T, Lebeau, A, Muratet, M, Simmons, B, Kloske, D, Sieja, S, Hudson, J, Sethupathy, P, Brownstein, M, Bhat, N, Lazar, J, Jacob, H, Gruber, CE, Smith, MR, McPherson, J, Garcia, AM, Gunaratne, PH, Wu, J, Muzny, D, Gibbs, RA, Young, AC, Bouffard, GG, Blakesley, RW, Mullikin, J, Green, ED, Dickson, MC, Rodriguez, AC, Grimwood, J, Schmutz, J, Myers, RM, Hirst, M, Zeng, T, Tse, K, Moksa, M, Deng, M, Ma, K, Mah, D, Pang, J, Taylor, G, Chuah, E, Deng, A, Fichter, K, Go, A, Lee, S, Wang, J, Griffith, M, Morin, R, Moore, RA, Mayo, M, Munro, S, Wagner, S, Jones, SJM, Holt, RA, Marra, MA, Lu, S, Yang, S, Hartigan, J, Graf, M, Wagner, R, Letovksy, S, Pulido, JC, Robison, K, Esposito, D, Hartley, J, Wall, VE, Hopkins, RF, Ohara, O & Wiemann, S 2009, 'The completion of the Mammalian Gene Collection', Genome Research, vol. 19, no. 12, pp. 2324-2333. https://doi.org/10.1101/gr.095976.109
    Temple G, Gerhard DS, Rasooly R, Feingold EA, Good PJ, Robinson C et al. The completion of the Mammalian Gene Collection. Genome Research. 2009 Dec 1;19(12):2324-2333. https://doi.org/10.1101/gr.095976.109
    Temple, Gary ; Gerhard, Daniela S. ; Rasooly, Rebekah ; Feingold, Elise A. ; Good, Peter J. ; Robinson, Cristen ; Mandich, Allison ; Derge, Jeffrey G. ; Lewis, Jeanne ; Shoaf, Debonny ; Collins, Francis S. ; Jang, Wonhee ; Wagner, Lukas ; Shenmen, Carolyn M. ; Misquitta, Leonie ; Schaefer, Carl F. ; Buetow, Kenneth H. ; Bonner, Tom I. ; Yankie, Linda ; Ward, Ming ; Phan, Lon ; Astashyn, Alex ; Brown, Garth ; Farrell, Catherine ; Hart, Jennifer ; Landrum, Melissa ; Maidak, Bonnie L. ; Murphy, Michael ; Murphy, Terence ; Rajput, Bhanu ; Riddick, Lillian ; Webb, David ; Weber, Janet ; Wu, Wendy ; Pruitt, Kim D. ; Maglott, Donna ; Siepel, Adam ; Brejova, Brona ; Diekhans, Mark ; Harte, Rachel ; Baertsch, Robert ; Kent, Jim ; Haussler, David ; Brent, Michael ; Langton, Laura ; Comstock, Charles L.G. ; Stevens, Michael ; Wei, Chaochun ; Van Baren, Marijke J. ; Salehi-Ashtiani, Kourosh ; Murray, Ryan R. ; Ghamsari, Lila ; Mello, Elizabeth ; Lin, Chenwei ; Pennacchio, Christa ; Schreiber, Kirsten ; Shapiro, Nicole ; Marsh, Amber ; Pardes, Elizabeth ; Moore, Troy ; Lebeau, Anita ; Muratet, Mike ; Simmons, Blake ; Kloske, David ; Sieja, Stephanie ; Hudson, James ; Sethupathy, Praveen ; Brownstein, Michael ; Bhat, Narayan ; Lazar, Joseph ; Jacob, Howard ; Gruber, Chris E. ; Smith, Mark R. ; McPherson, John ; Garcia, Angela M. ; Gunaratne, Preethi H. ; Wu, Jiaqian ; Muzny, Donna ; Gibbs, Richard A. ; Young, Alice C. ; Bouffard, Gerard G. ; Blakesley, Robert W. ; Mullikin, Jim ; Green, Eric D. ; Dickson, Mark C. ; Rodriguez, Alex C. ; Grimwood, Jane ; Schmutz, Jeremy ; Myers, Richard M. ; Hirst, Martin ; Zeng, Thomas ; Tse, Kane ; Moksa, Michelle ; Deng, Merinda ; Ma, Kevin ; Mah, Diana ; Pang, Johnson ; Taylor, Greg ; Chuah, Eric ; Deng, Athena ; Fichter, Keith ; Go, Anne ; Lee, Stephanie ; Wang, Jing ; Griffith, Malachi ; Morin, Ryan ; Moore, Richard A. ; Mayo, Michael ; Munro, Sarah ; Wagner, Susan ; Jones, Steven J.M. ; Holt, Robert A. ; Marra, Marco A. ; Lu, Sun ; Yang, Shuwei ; Hartigan, James ; Graf, Marcus ; Wagner, Ralf ; Letovksy, Stanley ; Pulido, Jacqueline C. ; Robison, Keith ; Esposito, Dominic ; Hartley, James ; Wall, Vanessa E. ; Hopkins, Ralph F. ; Ohara, Osamu ; Wiemann, Stefan. / The completion of the Mammalian Gene Collection. In: Genome Research. 2009 ; Vol. 19, No. 12. pp. 2324-2333.
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    AU - Griffith, Malachi

    AU - Morin, Ryan

    AU - Moore, Richard A.

    AU - Mayo, Michael

    AU - Munro, Sarah

    AU - Wagner, Susan

    AU - Jones, Steven J.M.

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    AU - Marra, Marco A.

    AU - Lu, Sun

    AU - Yang, Shuwei

    AU - Hartigan, James

    AU - Graf, Marcus

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    AU - Letovksy, Stanley

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