The coat morphogenetic protein SpoVID is necessary for spore encasement in Bacillus subtilis

Katherine H. Wang, Anabela L. Isidro, Lia Domingues, Haig A. Eskandarian, Peter T. McKenney, Kevin Drew, Paul Grabowski, Ming Hsiu Chua, Samantha N. Barry, Michelle Guan, Richard Bonneau, Adriano O. Henriques, Patrick Eichenberger

Research output: Contribution to journalArticle

Abstract

Endospores formed by Bacillus subtilis are encased in a tough protein shell known as the coat, which consists of at least 70 different proteins. We investigated the process of spore coat morphogenesis using a library of 40 coat proteins fused to green fluorescent protein and demonstrate that two successive steps can be distinguished in coat assembly. The first step, initial localization of proteins to the spore surface, is dependent on the coat morphogenetic proteins SpoIVA and SpoVM. The second step, spore encasement, requires a third protein, SpoVID. We show that in spoVID mutant cells, most coat proteins assembled into a cap at one side of the developing spore but failed to migrate around and encase it. We also found that SpoIVA directly interacts with SpoVID. A domain analysis revealed that the N-terminus of SpoVID is required for encasement and is a structural homologue of a virion protein, whereas the C-terminus is necessary for the interaction with SpoIVA. Thus, SpoVM, SpoIVA and SpoVID are recruited to the spore surface in a concerted manner and form a tripartite machine that drives coat formation and spore encasement. Journal compilation

Original languageEnglish (US)
Pages (from-to)634-649
Number of pages16
JournalMolecular Microbiology
Volume74
Issue number3
DOIs
StatePublished - Nov 2009

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Capsid Proteins
Bacillus subtilis
Spores
Proteins
Glycocalyx
Protein C
Green Fluorescent Proteins
Morphogenesis
Virion

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The coat morphogenetic protein SpoVID is necessary for spore encasement in Bacillus subtilis. / Wang, Katherine H.; Isidro, Anabela L.; Domingues, Lia; Eskandarian, Haig A.; McKenney, Peter T.; Drew, Kevin; Grabowski, Paul; Chua, Ming Hsiu; Barry, Samantha N.; Guan, Michelle; Bonneau, Richard; Henriques, Adriano O.; Eichenberger, Patrick.

In: Molecular Microbiology, Vol. 74, No. 3, 11.2009, p. 634-649.

Research output: Contribution to journalArticle

Wang, KH, Isidro, AL, Domingues, L, Eskandarian, HA, McKenney, PT, Drew, K, Grabowski, P, Chua, MH, Barry, SN, Guan, M, Bonneau, R, Henriques, AO & Eichenberger, P 2009, 'The coat morphogenetic protein SpoVID is necessary for spore encasement in Bacillus subtilis', Molecular Microbiology, vol. 74, no. 3, pp. 634-649. https://doi.org/10.1111/j.1365-2958.2009.06886.x
Wang, Katherine H. ; Isidro, Anabela L. ; Domingues, Lia ; Eskandarian, Haig A. ; McKenney, Peter T. ; Drew, Kevin ; Grabowski, Paul ; Chua, Ming Hsiu ; Barry, Samantha N. ; Guan, Michelle ; Bonneau, Richard ; Henriques, Adriano O. ; Eichenberger, Patrick. / The coat morphogenetic protein SpoVID is necessary for spore encasement in Bacillus subtilis. In: Molecular Microbiology. 2009 ; Vol. 74, No. 3. pp. 634-649.
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