The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription

Piergiorgio Percipalle, Nathalie Fomproix, Erica Cavellán, Renate Voit, Georg Reimer, Tim Krüger, Johan Thyberg, Ulrich Scheer, Ingrid Grummt, Ann Kristin Östlund Farrants

    Research output: Contribution to journalArticle

    Abstract

    Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein - protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.

    Original languageEnglish (US)
    Pages (from-to)525-530
    Number of pages6
    JournalEMBO Reports
    Volume7
    Issue number5
    DOIs
    StatePublished - May 1 2006

    Fingerprint

    Williams Syndrome
    RNA Polymerase I
    Chromatin Assembly and Disassembly
    Transcription
    Myosins
    Chromatin
    Actins
    Transcription Factors
    Genes
    Multiprotein Complexes
    Ribosomal RNA
    Chromatin Immunoprecipitation
    RNA Precursors
    Regulator Genes
    Fractionation
    Nuclear Proteins
    RNA Interference
    Ribosomal DNA
    rRNA Genes
    Assays

    Keywords

    • Chromatin remodelling complexes
    • Nuclear myosin 1
    • Pol I transcription
    • rDNA
    • SNF2h
    • WSTF

    ASJC Scopus subject areas

    • Genetics
    • Cell Biology

    Cite this

    Percipalle, P., Fomproix, N., Cavellán, E., Voit, R., Reimer, G., Krüger, T., ... Östlund Farrants, A. K. (2006). The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription. EMBO Reports, 7(5), 525-530. https://doi.org/10.1038/sj.embor.7400657

    The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription. / Percipalle, Piergiorgio; Fomproix, Nathalie; Cavellán, Erica; Voit, Renate; Reimer, Georg; Krüger, Tim; Thyberg, Johan; Scheer, Ulrich; Grummt, Ingrid; Östlund Farrants, Ann Kristin.

    In: EMBO Reports, Vol. 7, No. 5, 01.05.2006, p. 525-530.

    Research output: Contribution to journalArticle

    Percipalle, P, Fomproix, N, Cavellán, E, Voit, R, Reimer, G, Krüger, T, Thyberg, J, Scheer, U, Grummt, I & Östlund Farrants, AK 2006, 'The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription', EMBO Reports, vol. 7, no. 5, pp. 525-530. https://doi.org/10.1038/sj.embor.7400657
    Percipalle, Piergiorgio ; Fomproix, Nathalie ; Cavellán, Erica ; Voit, Renate ; Reimer, Georg ; Krüger, Tim ; Thyberg, Johan ; Scheer, Ulrich ; Grummt, Ingrid ; Östlund Farrants, Ann Kristin. / The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription. In: EMBO Reports. 2006 ; Vol. 7, No. 5. pp. 525-530.
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