The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription

Piergiorgio Percipalle, Nathalie Fomproix, Erica Cavellán, Renate Voit, Georg Reimer, Tim Krüger, Johan Thyberg, Ulrich Scheer, Ingrid Grummt, Ann Kristin Östlund Farrants

Research output: Contribution to journalArticle

Abstract

Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein - protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.

Original languageEnglish (US)
Pages (from-to)525-530
Number of pages6
JournalEMBO Reports
Volume7
Issue number5
DOIs
StatePublished - May 1 2006

Fingerprint

Williams Syndrome
RNA Polymerase I
Chromatin Assembly and Disassembly
Transcription
Myosins
Chromatin
Actins
Transcription Factors
Genes
Multiprotein Complexes
Ribosomal RNA
Chromatin Immunoprecipitation
RNA Precursors
Regulator Genes
Fractionation
Nuclear Proteins
RNA Interference
Ribosomal DNA
rRNA Genes
Assays

Keywords

  • Chromatin remodelling complexes
  • Nuclear myosin 1
  • Pol I transcription
  • rDNA
  • SNF2h
  • WSTF

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription. / Percipalle, Piergiorgio; Fomproix, Nathalie; Cavellán, Erica; Voit, Renate; Reimer, Georg; Krüger, Tim; Thyberg, Johan; Scheer, Ulrich; Grummt, Ingrid; Östlund Farrants, Ann Kristin.

In: EMBO Reports, Vol. 7, No. 5, 01.05.2006, p. 525-530.

Research output: Contribution to journalArticle

Percipalle, P, Fomproix, N, Cavellán, E, Voit, R, Reimer, G, Krüger, T, Thyberg, J, Scheer, U, Grummt, I & Östlund Farrants, AK 2006, 'The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription', EMBO Reports, vol. 7, no. 5, pp. 525-530. https://doi.org/10.1038/sj.embor.7400657
Percipalle, Piergiorgio ; Fomproix, Nathalie ; Cavellán, Erica ; Voit, Renate ; Reimer, Georg ; Krüger, Tim ; Thyberg, Johan ; Scheer, Ulrich ; Grummt, Ingrid ; Östlund Farrants, Ann Kristin. / The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription. In: EMBO Reports. 2006 ; Vol. 7, No. 5. pp. 525-530.
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