THE BEHAVIOR OF DYE‐MUCOPOLYSACCHARIDE COMPLEXES—I. SPECTROSCOPIC STUDIES OF THE SYSTEM CHONDROITIN SULFATE A‐ACRIDINE ORANGE

M. Salter, P. Gentilcore, R. S. Fager, G. P.P. Kuntz, E. W. Abrahamson, J. M. Menter, S. S. West

    Research output: Contribution to journalArticle

    Abstract

    Abstract—Absorption, fluorescence, and circular dichroism (CD) spectra have been obtained for a mucopolysaccharide—cationic dye complex. The dye acridine orange exhibits a blue shift in its absorption maximum and a red shift in its fluorescence maximum when mixed with chondroitin sulfate A in aqueous solution. The spectral shifts of the dye appear to be reversed by addition of salt, divalent cations being more effective than monovalent cations. The complex exhibits induced optical activity in the visible absorption spectrum of acridine orange. The biphasic nature of the CD curve is compatible with interpretations involving chromophore coupling.

    Original languageEnglish (US)
    Pages (from-to)59-63
    Number of pages5
    JournalPhotochemistry and photobiology
    Volume20
    Issue number1
    DOIs
    StatePublished - Jul 1974

      Fingerprint

    ASJC Scopus subject areas

    • Biochemistry
    • Physical and Theoretical Chemistry

    Cite this