The behavior of dye mucopolysaccharide complexes. I. Spectroscopic studies of the system chondroitin sulfate a acridine orange

Mary Cowman, P. Gentilcore, R. S. Fager

Research output: Contribution to journalArticle

Abstract

Absorption, fluorescence, and circular dichroism (CD) spectra have been obtained for a mucopolysaccharide cationic dye complex. The dye acridine orange exhibits a blue shift in its absorption maximum and a red shift in its fluorescence maximum when mixed with chondroitin sulfate A in aqueous solution. The spectral shifts of the dye appear to be reversed by addition of salt, divalent cations being more effective than monovalent cations. The complex exhibits induced optical activity in the visible absorption spectrum of acridine orange. The biphasic nature of the CD curve is compatible with interpretations involving chromophore coupling.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalPhotochemistry and Photobiology
Volume20
Issue number1
StatePublished - 1974

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Acridine Orange
Chondroitin Sulfates
Glycosaminoglycans
sulfates
Coloring Agents
dyes
Circular Dichroism
dichroism
Fluorescence
Optical Rotation
cations
Monovalent Cations
fluorescence
optical activity
Divalent Cations
Chromophores
visible spectrum
blue shift
red shift
chromophores

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

The behavior of dye mucopolysaccharide complexes. I. Spectroscopic studies of the system chondroitin sulfate a acridine orange. / Cowman, Mary; Gentilcore, P.; Fager, R. S.

In: Photochemistry and Photobiology, Vol. 20, No. 1, 1974, p. 59-63.

Research output: Contribution to journalArticle

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