The antiproliferative role of ERG K+ channels in rat osteoblastic cells

Leonardo Hernandez, Ki Ho Park, Shi Qing Cai, Ling Qin, Nicola Partridge, Federico Sesti

Research output: Contribution to journalArticle

Abstract

We report on the role of K+ currents in the mechanisms regulating the proliferation of UMR 106-01 osteoblastic osteosarcoma cells. Electrophysiological analysis showed that UMR 106-01 cells produce robust K+ currents that can be pharmacologically separated into two major components: a E-4031-susceptible current, I E-4031, and a tetraethylammonium (TEA)-susceptible component, I TEA. Western blot and RT-PCR analysis showed that I E-4031 is produced by ether a go-go (eag)-related channels (ERG). Incubation of the cells with E-4031 enhanced their proliferation by 80%. Application of E-4031 in the bath solution did not induce instantaneous changes in the membrane resting potential or in the level of cytosolic calcium; however, the cells were slightly depolarized and the calcium content was significantly increased upon prolonged incubation with the compound. Taken together these findings indicate that ERG channels can impair cell proliferation. This is a novel finding that underscores new modes of regulation of mitosis by voltage-gated K+ channels and provides an unexpected insight into the current view of the mechanisms governing bone tissue proliferation.

Original languageEnglish (US)
Pages (from-to)199-208
Number of pages10
JournalCell Biochemistry and Biophysics
Volume47
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Rats
Tetraethylammonium
Calcium
Voltage-Gated Potassium Channels
Cell proliferation
Osteosarcoma
Baths
Mitosis
Ether
Membrane Potentials
Bone
Western Blotting
Cells
Cell Proliferation
E 4031
Tissue
Membranes
Bone and Bones
Polymerase Chain Reaction

Keywords

  • Bone
  • Potassium channel
  • Proliferation

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Biophysics

Cite this

The antiproliferative role of ERG K+ channels in rat osteoblastic cells. / Hernandez, Leonardo; Park, Ki Ho; Cai, Shi Qing; Qin, Ling; Partridge, Nicola; Sesti, Federico.

In: Cell Biochemistry and Biophysics, Vol. 47, No. 2, 02.2007, p. 199-208.

Research output: Contribution to journalArticle

Hernandez, Leonardo ; Park, Ki Ho ; Cai, Shi Qing ; Qin, Ling ; Partridge, Nicola ; Sesti, Federico. / The antiproliferative role of ERG K+ channels in rat osteoblastic cells. In: Cell Biochemistry and Biophysics. 2007 ; Vol. 47, No. 2. pp. 199-208.
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