Abstract
We report on the role of K+ currents in the mechanisms regulating the proliferation of UMR 106-01 osteoblastic osteosarcoma cells. Electrophysiological analysis showed that UMR 106-01 cells produce robust K+ currents that can be pharmacologically separated into two major components: a E-4031-susceptible current, I E-4031, and a tetraethylammonium (TEA)-susceptible component, I TEA. Western blot and RT-PCR analysis showed that I E-4031 is produced by ether a go-go (eag)-related channels (ERG). Incubation of the cells with E-4031 enhanced their proliferation by 80%. Application of E-4031 in the bath solution did not induce instantaneous changes in the membrane resting potential or in the level of cytosolic calcium; however, the cells were slightly depolarized and the calcium content was significantly increased upon prolonged incubation with the compound. Taken together these findings indicate that ERG channels can impair cell proliferation. This is a novel finding that underscores new modes of regulation of mitosis by voltage-gated K+ channels and provides an unexpected insight into the current view of the mechanisms governing bone tissue proliferation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 199-208 |
| Number of pages | 10 |
| Journal | Cell Biochemistry and Biophysics |
| Volume | 47 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2007 |
Fingerprint
Keywords
- Bone
- Potassium channel
- Proliferation
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Biophysics
Cite this
The antiproliferative role of ERG K+ channels in rat osteoblastic cells. / Hernandez, Leonardo; Park, Ki Ho; Cai, Shi Qing; Qin, Ling; Partridge, Nicola; Sesti, Federico.
In: Cell Biochemistry and Biophysics, Vol. 47, No. 2, 02.2007, p. 199-208.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The antiproliferative role of ERG K+ channels in rat osteoblastic cells
AU - Hernandez, Leonardo
AU - Park, Ki Ho
AU - Cai, Shi Qing
AU - Qin, Ling
AU - Partridge, Nicola
AU - Sesti, Federico
PY - 2007/2
Y1 - 2007/2
N2 - We report on the role of K+ currents in the mechanisms regulating the proliferation of UMR 106-01 osteoblastic osteosarcoma cells. Electrophysiological analysis showed that UMR 106-01 cells produce robust K+ currents that can be pharmacologically separated into two major components: a E-4031-susceptible current, I E-4031, and a tetraethylammonium (TEA)-susceptible component, I TEA. Western blot and RT-PCR analysis showed that I E-4031 is produced by ether a go-go (eag)-related channels (ERG). Incubation of the cells with E-4031 enhanced their proliferation by 80%. Application of E-4031 in the bath solution did not induce instantaneous changes in the membrane resting potential or in the level of cytosolic calcium; however, the cells were slightly depolarized and the calcium content was significantly increased upon prolonged incubation with the compound. Taken together these findings indicate that ERG channels can impair cell proliferation. This is a novel finding that underscores new modes of regulation of mitosis by voltage-gated K+ channels and provides an unexpected insight into the current view of the mechanisms governing bone tissue proliferation.
AB - We report on the role of K+ currents in the mechanisms regulating the proliferation of UMR 106-01 osteoblastic osteosarcoma cells. Electrophysiological analysis showed that UMR 106-01 cells produce robust K+ currents that can be pharmacologically separated into two major components: a E-4031-susceptible current, I E-4031, and a tetraethylammonium (TEA)-susceptible component, I TEA. Western blot and RT-PCR analysis showed that I E-4031 is produced by ether a go-go (eag)-related channels (ERG). Incubation of the cells with E-4031 enhanced their proliferation by 80%. Application of E-4031 in the bath solution did not induce instantaneous changes in the membrane resting potential or in the level of cytosolic calcium; however, the cells were slightly depolarized and the calcium content was significantly increased upon prolonged incubation with the compound. Taken together these findings indicate that ERG channels can impair cell proliferation. This is a novel finding that underscores new modes of regulation of mitosis by voltage-gated K+ channels and provides an unexpected insight into the current view of the mechanisms governing bone tissue proliferation.
KW - Bone
KW - Potassium channel
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=34548348372&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548348372&partnerID=8YFLogxK
U2 - 10.1007/s12013-007-0006-9
DO - 10.1007/s12013-007-0006-9
M3 - Article
C2 - 17652772
AN - SCOPUS:34548348372
VL - 47
SP - 199
EP - 208
JO - Cell Biochemistry and Biophysics
JF - Cell Biochemistry and Biophysics
SN - 1085-9195
IS - 2
ER -