The activity of the Drosophila morphogenetic protein Bicoid is inhibited by a domain located outside its homeodomain

Chen Zhao, Allen York, Fan Yang, David J. Forsthoefel, Vrushank Dave, Dechen Fu, Dongyi Zhang, Maria S. Corado, Stephen Small, Mark A. Seeger, Jun Ma

Research output: Contribution to journalArticle

Abstract

The Drosophila morphogenetic protein Bicoid (Bcd) is a homeodomain-containing activator that stimulates the expression of target genes during early embryonic development. We demonstrate that a small domain of Bcd located immediately N-terminally of the homeodomain represses its own activity in Drosophila cells. This domain, referred to as a self-inhibitory domain, works as an independent module that does not rely on any other sequences of Bcd and can repress the activity of heterologous activators. We further show that this domain of Bcd does not affect its properties of DNA binding or subcellular distribution. A Bcd derivative with point mutations in the self-inhibitory domain severely affects pattern formation and target gene expression in Drosophila embryos. We also provide evidence to suggest that the action of the self-inhibitory domain requires a Drosophila co-factor(s), other than CtBP or dSAP18. Our results suggest that proper action of Bcd as a transcriptional activator and molecular morphogen during embryonic development is dependent on the downregulation of its own activity through an interaction with a novel co-repressor(s) or complex(es).

Original languageEnglish (US)
Pages (from-to)1669-1680
Number of pages12
JournalDevelopment
Volume129
Issue number7
StatePublished - 2002

Fingerprint

Drosophila
Embryonic Development
Gene Expression
Co-Repressor Proteins
Point Mutation
Down-Regulation
Embryonic Structures
DNA
Drosophila bicoid protein

Keywords

  • Bicoid
  • Drosophila
  • Transcription

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Zhao, C., York, A., Yang, F., Forsthoefel, D. J., Dave, V., Fu, D., ... Ma, J. (2002). The activity of the Drosophila morphogenetic protein Bicoid is inhibited by a domain located outside its homeodomain. Development, 129(7), 1669-1680.

The activity of the Drosophila morphogenetic protein Bicoid is inhibited by a domain located outside its homeodomain. / Zhao, Chen; York, Allen; Yang, Fan; Forsthoefel, David J.; Dave, Vrushank; Fu, Dechen; Zhang, Dongyi; Corado, Maria S.; Small, Stephen; Seeger, Mark A.; Ma, Jun.

In: Development, Vol. 129, No. 7, 2002, p. 1669-1680.

Research output: Contribution to journalArticle

Zhao, C, York, A, Yang, F, Forsthoefel, DJ, Dave, V, Fu, D, Zhang, D, Corado, MS, Small, S, Seeger, MA & Ma, J 2002, 'The activity of the Drosophila morphogenetic protein Bicoid is inhibited by a domain located outside its homeodomain', Development, vol. 129, no. 7, pp. 1669-1680.
Zhao C, York A, Yang F, Forsthoefel DJ, Dave V, Fu D et al. The activity of the Drosophila morphogenetic protein Bicoid is inhibited by a domain located outside its homeodomain. Development. 2002;129(7):1669-1680.
Zhao, Chen ; York, Allen ; Yang, Fan ; Forsthoefel, David J. ; Dave, Vrushank ; Fu, Dechen ; Zhang, Dongyi ; Corado, Maria S. ; Small, Stephen ; Seeger, Mark A. ; Ma, Jun. / The activity of the Drosophila morphogenetic protein Bicoid is inhibited by a domain located outside its homeodomain. In: Development. 2002 ; Vol. 129, No. 7. pp. 1669-1680.
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