The absence of the calcium-buffering protein calbindin is associated with faster age-related decline in hippocampal metabolism

Herman Moreno, Nesha S. Burghardt, Daniel Vela-Duarte, James Masciotti, Fan Hua, Andre Fenton, Beat Schwaller, Scott A. Small

Research output: Contribution to journalArticle

Abstract

Although reductions in the expression of the calcium-buffering proteins calbindin D-28K (CB) and parvalbumin (PV) have been observed in the aging brain, it is unknown whether these changes contribute to age-related hippocampal dysfunction. To address this issue, we measured basal hippocampal metabolism and hippocampal structure across the lifespan of C57BL/6J, calbindin D-28k knockout (CBKO) and parvalbumin knockout (PVKO) mice. Basal metabolism was estimated using steady state relative cerebral blood volume (rCBV), which is a variant of fMRI that provides the highest spatial resolution, optimal for the analysis of individual subregions of the hippocampal formation. We found that like primates, normal aging in C57BL/6J mice is characterized by an age-dependent decline in rCBV-estimated dentate gyrus (DG) metabolism. Although abnormal hippocampal fMRI signals were observed in CBKO and PVKO mice, only CBKO mice showed accelerated age-dependent decline of rCBV-estimated metabolism in the DG. We also found age-independent structural changes in CBKO mice, which included an enlarged hippocampus and neocortex as well as global brain hypertrophy. These metabolic and structural changes in CBKO mice correlated with a deficit in hippocampus-dependent learning in the active place avoidance task. Our results suggest that the decrease in CB that occurs during normal aging is involved in age-related hippocampal metabolic decline. Our findings also illustrate the value of using multiple MRI techniques in transgenic mice to investigate mechanisms involved in the functional and structural changes that occur during aging.

Original languageEnglish (US)
Pages (from-to)1107-1120
Number of pages14
JournalHippocampus
Volume22
Issue number5
DOIs
StatePublished - May 2012

Fingerprint

Calbindins
Knockout Mice
Calcium
Parvalbumins
Basal Metabolism
Hippocampus
Proteins
Dentate Gyrus
Magnetic Resonance Imaging
Problem-Based Learning
Neocortex
Brain
Inbred C57BL Mouse
Hypertrophy
Primates
Transgenic Mice
Cerebral Blood Volume

Keywords

  • Aging
  • CBKO
  • CBV
  • FMRI (functional magnetic resonance imaging)
  • Parvalbumin

ASJC Scopus subject areas

  • Cognitive Neuroscience

Cite this

The absence of the calcium-buffering protein calbindin is associated with faster age-related decline in hippocampal metabolism. / Moreno, Herman; Burghardt, Nesha S.; Vela-Duarte, Daniel; Masciotti, James; Hua, Fan; Fenton, Andre; Schwaller, Beat; Small, Scott A.

In: Hippocampus, Vol. 22, No. 5, 05.2012, p. 1107-1120.

Research output: Contribution to journalArticle

Moreno, H, Burghardt, NS, Vela-Duarte, D, Masciotti, J, Hua, F, Fenton, A, Schwaller, B & Small, SA 2012, 'The absence of the calcium-buffering protein calbindin is associated with faster age-related decline in hippocampal metabolism', Hippocampus, vol. 22, no. 5, pp. 1107-1120. https://doi.org/10.1002/hipo.20957
Moreno, Herman ; Burghardt, Nesha S. ; Vela-Duarte, Daniel ; Masciotti, James ; Hua, Fan ; Fenton, Andre ; Schwaller, Beat ; Small, Scott A. / The absence of the calcium-buffering protein calbindin is associated with faster age-related decline in hippocampal metabolism. In: Hippocampus. 2012 ; Vol. 22, No. 5. pp. 1107-1120.
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