Tendamistat surface accessibility to the TEMPOL paramagnetic probe

Maria Scarselli, Andrea Bernini, Claudia Segoni, Henriette Molinari, Gennaro Esposito, Arthur M. Lesk, Franco Laschi, Pierandrea Temussi, Neri Niccolai

    Research output: Contribution to journalArticle

    Abstract

    TEMPOL, the soluble spin-label 4-hydroxy-2,2,6,6-tetramethyl-piperidine-1-oxyl, has been used to determine the surface characteristics of tendamistat, a small protein with a well-characterised structure both in solution and in the crystal. A good correlation has been found between predicted regions of exposed protein surface and the intensity attenuations induced by the probe on 2D NMR TOCSY cross peaks of tendamistat in the paramagnetic water solution. All the high paramagnetic effects have been interpreted in terms of more efficient competition of TEMPOL with water molecules at some surface positions. The active site of tendamistat coincides with the largest surface patch accessible to the probe. A strong hydration of protein N and C termini can also be suggested by this structural approach, as these locations exhibit reduced paramagnetic pertubations. Provided that the solution structure is known, the use of this paramagnetic probe seems to be well suited to delineate the dynamic behaviour of the protein surface and, more generally, to gain relevant information about the molecular presentation processes.

    Original languageEnglish (US)
    Pages (from-to)125-133
    Number of pages9
    JournalJournal of Biomolecular NMR
    Volume15
    Issue number2
    DOIs
    StatePublished - Dec 1 1999

    Fingerprint

    Membrane Proteins
    Spin Labels
    Water
    Protein C
    Hydration
    Catalytic Domain
    Proteins
    Nuclear magnetic resonance
    Crystals
    Molecules
    tendamistate
    2,2,6,6-tetramethyl-1-piperidine

    Keywords

    • Molecular presentation
    • Protein structure
    • Spin-labels
    • Surface accessibility
    • Tendamistat

    ASJC Scopus subject areas

    • Spectroscopy
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry

    Cite this

    Scarselli, M., Bernini, A., Segoni, C., Molinari, H., Esposito, G., Lesk, A. M., ... Niccolai, N. (1999). Tendamistat surface accessibility to the TEMPOL paramagnetic probe. Journal of Biomolecular NMR, 15(2), 125-133. https://doi.org/10.1023/A:1008319507565

    Tendamistat surface accessibility to the TEMPOL paramagnetic probe. / Scarselli, Maria; Bernini, Andrea; Segoni, Claudia; Molinari, Henriette; Esposito, Gennaro; Lesk, Arthur M.; Laschi, Franco; Temussi, Pierandrea; Niccolai, Neri.

    In: Journal of Biomolecular NMR, Vol. 15, No. 2, 01.12.1999, p. 125-133.

    Research output: Contribution to journalArticle

    Scarselli, M, Bernini, A, Segoni, C, Molinari, H, Esposito, G, Lesk, AM, Laschi, F, Temussi, P & Niccolai, N 1999, 'Tendamistat surface accessibility to the TEMPOL paramagnetic probe', Journal of Biomolecular NMR, vol. 15, no. 2, pp. 125-133. https://doi.org/10.1023/A:1008319507565
    Scarselli M, Bernini A, Segoni C, Molinari H, Esposito G, Lesk AM et al. Tendamistat surface accessibility to the TEMPOL paramagnetic probe. Journal of Biomolecular NMR. 1999 Dec 1;15(2):125-133. https://doi.org/10.1023/A:1008319507565
    Scarselli, Maria ; Bernini, Andrea ; Segoni, Claudia ; Molinari, Henriette ; Esposito, Gennaro ; Lesk, Arthur M. ; Laschi, Franco ; Temussi, Pierandrea ; Niccolai, Neri. / Tendamistat surface accessibility to the TEMPOL paramagnetic probe. In: Journal of Biomolecular NMR. 1999 ; Vol. 15, No. 2. pp. 125-133.
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