Temporal requirement for hedgehog signaling in ventral telencephalic patterning

Marc Fuccillo, Murielle Rallu, Andrew P. McMahon, Gordon Fishell

Research output: Contribution to journalArticle

Abstract

Hedgehog signaling is required for multiple aspects of brain development, including growth, the establishment of both dorsal and ventral midline patterning and the generation of specific cell types such as oligodendrocytes and interneurons. To identify more precisely when during development hedgehog signaling mediates these events, we directed the removal of hedgehog signaling within the brain by embryonic day 9 of development, using a FoxG1Cre driver line to mediate the removal of a conditional smoothened null allele. We observed a loss of ventral telencephalic patterning that appears to result from an initial lack of specification of these structures rather than by changes in proliferation or cell death. A further consequence of the removal of smoothened in these mice is the near absence of both oligodendrocytes and interneurons. Surprisingly, the dorsal midline appears to be patterned normally in these mutants. Together with previous analyses, the present results demonstrate that hedgehog signaling in the period between E9.0 and E12 is essential for the patterning of ventral regions and the generation of cell types that are thought to largely arise from them.

Original languageEnglish (US)
Pages (from-to)5031-5040
Number of pages10
JournalDevelopment
Volume131
Issue number20
DOIs
StatePublished - Oct 1 2004

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Telencephalon
Hedgehogs
Oligodendroglia
Interneurons
Brain
Growth and Development
Cell Death
Alleles

Keywords

  • Interneuron
  • Oligodendrocyte
  • Patterning
  • Sonic hedgehog
  • Telencephalon

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Temporal requirement for hedgehog signaling in ventral telencephalic patterning. / Fuccillo, Marc; Rallu, Murielle; McMahon, Andrew P.; Fishell, Gordon.

In: Development, Vol. 131, No. 20, 01.10.2004, p. 5031-5040.

Research output: Contribution to journalArticle

Fuccillo, Marc ; Rallu, Murielle ; McMahon, Andrew P. ; Fishell, Gordon. / Temporal requirement for hedgehog signaling in ventral telencephalic patterning. In: Development. 2004 ; Vol. 131, No. 20. pp. 5031-5040.
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